【英語(yǔ)名稱】:TNFRSF18 (Human) ELISA Kit
【中文名稱】:TNFRSF18 (人源) ELISA試劑盒
【產(chǎn)品類型】:免疫學(xué)試劑盒【供應(yīng)商】:Abnova
【產(chǎn)品貨號(hào)】:KA1719
【產(chǎn)品規(guī)格】:1 Kit
【背景資料】:TNFRSF18 (人源) ELISA試劑盒產(chǎn)品為Abnova旗下提供的高品質(zhì)試劑盒產(chǎn)品。作為多家國(guó)際知名試劑盒品牌的中國(guó)區(qū)域代理,艾美捷能為您提供更多TNFRSF18 (人源) ELISA試劑盒產(chǎn)品或相關(guān)產(chǎn)品的解決方案。
【保存建議】:請(qǐng)?jiān)诮邮盏絋NFRSF18 (人源) ELISA試劑盒產(chǎn)品后,按照產(chǎn)品操作說(shuō)明書對(duì)試劑盒內(nèi)不同組份進(jìn)行適當(dāng)?shù)谋4妗?/p>
【產(chǎn)品說(shuō)明】:點(diǎn)擊:TNFRSF18 (人源) ELISA試劑盒查看更詳細(xì)產(chǎn)品說(shuō)明,更多應(yīng)用、儲(chǔ)存、價(jià)格、貨期等信息請(qǐng)致電400-6800-868垂詢Abnova中國(guó)區(qū)域授權(quán)總代理艾美捷科技有限公司。
作為全世界最大的單克隆抗體和蛋白生產(chǎn)商,Abnova的金牌品質(zhì)使其成為全球最主要抗體品牌的供應(yīng)商,您可以在各個(gè)主要抗體品牌,如Abcam、Sigma、Novus、AbD等知名品牌的目錄上都能發(fā)現(xiàn)Abnova的抗體。作為Abnova的中國(guó)區(qū)域代理,艾美捷科技有限公司憑借豐富的專業(yè)經(jīng)驗(yàn),將一如既往的為中國(guó)區(qū)域客戶提供Abnova優(yōu)質(zhì)的產(chǎn)品和技術(shù)服務(wù),包括各種抗體、重組蛋白、抗體對(duì)、ELISA試劑盒、原位雜交熒光探針、蛋白定量以及可用于蛋白翻譯研究、診斷和制藥公司應(yīng)用的基礎(chǔ)型體外診斷設(shè)備等。
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樣品收集、處理及保存方法
樣品采集:收集標(biāo)本前必須清楚要檢測(cè)的成份是否足夠穩(wěn)定。對(duì)收集后當(dāng)天進(jìn)行檢測(cè)的標(biāo)本,儲(chǔ)存在4℃?zhèn)溆,如有特殊原因需要周期收集?biāo)本,將標(biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。避免反復(fù)凍融。標(biāo)本2-8℃可保存48小時(shí),-20℃可保存1個(gè)月。-70度可保存6個(gè)月。 部分激素類標(biāo)本需添加抑肽酶。人腫瘤壞死因子受體超家族成員18(TNFRSF18)ELISA試劑盒操作步驟:1. 使用前,將所有試劑充分混勻。不要使液體產(chǎn)生大量的泡沫,以免加樣時(shí)加入大量的氣泡,產(chǎn)生加樣上的誤差。2. 根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù)。每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔。每個(gè)樣品根據(jù)自己的數(shù)量來(lái)定,能使用復(fù)孔的盡量做復(fù)孔。3. 加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測(cè)樣品50ul于反應(yīng)孔內(nèi)。立即加入50ul的生物素標(biāo)記的抗體。蓋上膜板,輕輕振蕩混勻,37℃溫育1小時(shí)。4. 甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干。重復(fù)此操作3次。如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次。5. 每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘。6. 甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干。重復(fù)此操作3次。如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次。7. 每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘。避免光照。8. 取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測(cè)定結(jié)果。9. 在450nm波長(zhǎng)處測(cè)定各孔的OD值。人腫瘤壞死因子受體超家族成員18(TNFRSF18)ELISA試劑盒結(jié)果判斷與分析
1、儀器值:于波長(zhǎng)450nm的酶標(biāo)儀上讀取各孔的OD值
2、以吸光度OD值為縱坐標(biāo)(Y),相應(yīng)的待測(cè)物質(zhì)標(biāo)準(zhǔn)品濃度為橫坐標(biāo)(X),在坐標(biāo)紙上做得相應(yīng)的曲線,樣品的待測(cè)物質(zhì)含量可根據(jù)其OD值由標(biāo)準(zhǔn)曲線換算出相應(yīng)的濃度。
注意事項(xiàng)
產(chǎn)品簡(jiǎn)介: | |||||||||||||||||||
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武漢恒美液壓氣動(dòng)設(shè)備有限公司
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歡迎垂詢超凡機(jī)械盡在HF1800型摩托車破碎機(jī)F
鞏義市宏富機(jī)械制造有限公司研發(fā)部根據(jù)廢舊金屬回收市場(chǎng)的迫切需要,組織力量并結(jié)合國(guó)內(nèi)外客戶的具體應(yīng)用實(shí)際研制生產(chǎn)出新型、高效、節(jié)能、環(huán)保的新一代金屬破碎機(jī),具有操作簡(jiǎn)單方便、結(jié)構(gòu)緊促、產(chǎn)出量大等顯著優(yōu)點(diǎn)。該金屬破碎機(jī)主要運(yùn)用于大型廢品回收站、廢金屬回收公司等,可以將廢舊自行車、摩托車、廢舊電線、易拉罐、啤酒瓶蓋、鐵盒、油漆桶等金屬破碎成理想的顆粒,從而大大方便運(yùn)輸、加工和減少運(yùn)輸成本。用戶可根據(jù)所要加工原料的種類和對(duì)成品物料的要求(破碎顆粒大。┻x購(gòu)不同的機(jī)型和配置。宏富金屬破碎機(jī)憑借技術(shù)先進(jìn)、質(zhì)量可靠、價(jià)格公平的優(yōu)勢(shì)早已遠(yuǎn)銷南非、澳大利亞、俄羅斯等地并得到國(guó)內(nèi)外客戶的一致好評(píng)并。
HF1800型摩托車破碎機(jī)引爆耀眼商機(jī)
熱烈慶祝HF1800型摩托車破碎機(jī)成功銷往馬來(lái)西亞,這種破碎機(jī)是目前市場(chǎng)上比較大型的金屬破碎設(shè)備,具有扭力大,產(chǎn)量高,省動(dòng)力,耐磨和等優(yōu)點(diǎn)!HF1800摩托車破碎機(jī)的工作原理:是利用錘子擊打的基本原理,上料口帶有過(guò)濾式傳送帶,在高速大扭矩電機(jī)的驅(qū)動(dòng)下,主機(jī)轉(zhuǎn)子上的錘頭輪流擊打進(jìn)入容腔內(nèi)的待破碎物,通過(guò)襯板與錘頭之間形成的空間,將待破碎物撕裂成合乎規(guī)格的破碎物。
鞏義市宏富機(jī)械制造有限公司旨在為您提供更好的產(chǎn)品及最為經(jīng)濟(jì)合理的解決方案,我們的技術(shù)專家隨時(shí)為您提供技術(shù)幫助,并希望與您建立長(zhǎng)期的、追求更高價(jià)值的合作關(guān)系!