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    • orb101171HE4 antibody兔多克隆抗體

                  品牌:biorbyt

       類(lèi)型:Primary antibodies

       產(chǎn)品名稱:HE4 antibody            名稱:HE4 antibody 抗體 / 兔多克隆抗體

       貨號(hào):orb13596

       受體:Rabbit

       克隆能力:Polyclonal

       測(cè)試應(yīng)用:WB , IHC-P , IF/ICC

       規(guī)格:100ug、200ug

       適用種屬:Human, Mouse, Rat

      該公司產(chǎn)品分類(lèi): 產(chǎn)品目錄 試劑盒 蛋白 抗體

      2010_ENET_S嵌入式Ethernet通信接口模塊

      基本說(shuō)明;
      EtherNet/IP協(xié)議從設(shè)備通信功能;10/100M自適應(yīng)以太網(wǎng)接口;ARM7/44M處理器; 最多32路I/O通道;5V電源供電;用戶可進(jìn)行二次開(kāi)發(fā)。
      可用于:
      AC/DC驅(qū)動(dòng)器、馬達(dá)啟動(dòng)器;馬達(dá)控制;接近傳感器;氣動(dòng)閥; 運(yùn)動(dòng)控制;通用I/O器件; 繼電器、接觸器、斷路器 ;顯示設(shè)備;條形碼設(shè)備; 其它工業(yè)電氣和控制設(shè)備。
      工作環(huán)境溫度:-20 ~ 60 ℃ 相對(duì)濕度: 5 to 95% (無(wú)凝露)。
      符合EMC測(cè)試標(biāo)準(zhǔn);符合ODVA測(cè)試標(biāo)準(zhǔn)。
      本產(chǎn)品可根據(jù)用戶的要求為特定產(chǎn)品進(jìn)行定制設(shè)計(jì),定制可以增減功能單元,更節(jié)省成本或增強(qiáng)功能。輸入和輸出的數(shù)目和類(lèi)型可以進(jìn)行定制。

      賀利氏DS252/05J替代Thermo UV6000LP 氘燈108052

      介紹:賀利氏DS252/05J替代Thermo UV6000LP 氘燈108052德國(guó)賀利氏(Heraeus)UV6000LP氘燈歡迎來(lái)到沈陽(yáng)鎂匯科技有限公司,進(jìn)口氘燈全國(guó)總經(jīng)銷(xiāo):waters沃特斯氘燈、shimadzu島津氘燈、agilent安捷倫氘燈、pe氘燈、Hitachi日立氘燈、賀利氏氘燈Heraes氘燈、varian瓦里安氘燈、dionex戴安氘燈、beckman貝克曼氘燈、knauer諾爾氘燈、gilson氘燈、SSIAltex?氘燈、Thermo氘燈、英麟氘燈、Biotage氘燈、Isco氘燈、普析通用、福立氘燈、伍豐氘燈、創(chuàng)新通恒/北分瑞利氘燈等。購(gòu)買(mǎi)前請(qǐng)確認(rèn):儀器廠商、儀器/檢測(cè)器型號(hào)、原裝燈貨號(hào),原裝燈、置換燈的具體價(jià)格請(qǐng)致電本公司aaa我公司承諾,自氘燈到貨之日起,若安裝到設(shè)備提示能量值低或無(wú)法點(diǎn)亮,我公司負(fù)責(zé)更換新燈并承擔(dān)往返運(yùn)費(fèi)。到貨6個(gè)月內(nèi)為質(zhì)保期,若我公司出售的產(chǎn)品在質(zhì)保期內(nèi)出現(xiàn)能量值降低等狀況,我公司檢測(cè),若為氘燈質(zhì)量問(wèn)題,我公司負(fù)責(zé)更換新燈并承擔(dān)往返運(yùn)費(fèi)。沈陽(yáng)鎂匯科技有限公司地址:沈陽(yáng)市鐵西區(qū)北滑翔路21號(hào)電話:  傳真:手機(jī):aaa  QQ:aaa網(wǎng)址:aaa購(gòu)買(mǎi)地址:aaa?spm=a1z10.3-c-s.0.0.17e412a0AOxfPx
      該公司產(chǎn)品分類(lèi): Lear Sigler氘燈 LDC氘燈 Kontron氘燈 3Q認(rèn)證 Heraeus氘燈 MH系列氘燈 元素?zé)?/a> 氙燈 鎢燈 shodex色譜柱 PID燈 福立氘燈 伍豐氘燈 創(chuàng)新通恒/北分瑞利 Knauer氘燈 Gilson氘燈 Beckman Hitachi氘燈 Waters氘燈 Agilent氘燈

      希而科原裝進(jìn)口歐洲工控產(chǎn)品HELVAR?Digidim 458

       希而科原裝進(jìn)口歐洲工控產(chǎn)品 超快物流 特價(jià)供應(yīng)
      業(yè)務(wù)員:陳浩
      -----------------------------
      希而科工業(yè)控制設(shè)備(上海)有限公司
       
      電話:
      傳真:021-
      手機(jī):189 6458
      QQ:
      chenhao@
      www.
       
       
      品牌   型號(hào)ViscoTec Pumpen 4RI 15D 50l/h 2Mpa 
      Balluff GmbH BTL6-A-3801-2  
      BENDER IR425-D4-2 
      SIEMENS 3RV2011-4EA10 
      James Walker Deutschland?340*315*1000mm 
      SWKP3-10/P00 NR:8007375 SWKP3-10/P00 NR:8007376 
      6FX2007-1AD02 siemens 
      6EP1334-2BA01(6EP1334-2BA20) SIEMENS 
      TUENKERS A12 for V50.1 
      Vulkoprin Deutschland GmbH & Co. KG VK.B 125/50/325 
      : 
      SCHMERSAL SRB 202CA-24V  
      VUHZ SH8-M3/330_RD880(A)VW-SH8M3-A 
      JOST?TYP JD-50 T-NR.154038 M-NR.0260949 
      GRAEFF GMBH 24740 2XPT100/B/3 DINEN60751 7143 
      SCHUNK SRU+D 25-W-180-3-M-4-AS 
      6SY7010-0AB06 SIEMENS 
      HENGSTLER?RA58-P/1212EK.42TZDB 
      Reiff Technische Produkte GmbH GM-PU C 30*30 M8-8 453630  
      J+J Deutschland GmbH?MOD:L10 TYPE:J2 24 DC/AC 0.39A  
      SALMSON SHC20-134/1.85-T/A-E/S-1/OEN /01 No.066/67187  
      Karl Dungs GGW 3 A4 Au-M-MS9-V0-VS3 st-se 248673 01-100950 
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      6EP1 931-2EC21 SIEMENS 
      6SY7000-0AB18 SIEMENS 
       () BERNSTEIN SLK-FVTU24UC-55-ARX 
      RESATRON RSG10R1200-Y-3-G-V1-SS 
      EMG-1875 SV1-10/4/100/6  
      HP-TECHNIK VBHG-D-4-10 
      ITEM?ITEM 0.0.416.87 
      FGL3062 FGL3063 
       206.71.020.071 FIBRO  
      THIES THIES/GG25, TS904, 356/357G, 3582KG / h, serial number 144558, DO37, 9, D / G 0,69,8 bar 
      JANESX-3-60-TRA + TWTU-0014 + transmitter L = 250mm 0-200 ° C 
      Kistler?4075A50 
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      schmersal P/N??185.1048??00??TYPE??CC??8/S??11??12V 1.9A;AB??560 2??MILE FOG SIGNAL??AB??26 HALF MILE FOG SIGNAL??ML??300 LANTERN 
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      parker D81FSA32E4NXP037 
      FLEXLIFT FFRT-0245/12100630  
      Kniel?FPM27 a-nr??355-053-01 95770134 
      VICKERS Hydraulic Valve RELIEF VALVE DGMC2-3-AT-CW-BT-CW-41 
      SIEMENS 3RV1021-1EA15 2.8-4A 
      ITAS?NO.A50110452 
      Gestra BK15 PN25 DN40 
      6SY7010-0AA11 SIEMENS 
      7000-41131-000-0000 Murr 
      6ES7132-4BB31-0AB0 SIEMENS 
      AKO VF050.03X.33.30LA  
      Sick 1064201 AFM60A-TETA262144 ABSOLUTE ENCODER  
      JANE91-018-872-05 
      KOBOLD--D-71065 
      KUBLER 8.A02H.3532.1024 
      TF40-85/15578 
      L+B GEL8232B202 
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      ViscoTec Pumpen  
      Jungmichel Industrieelektronik GmbH?SI43 
      ITALWEBER S.p.A?CH10/6A 
      SUN--CBGH-LJV 
       TSS150/L M5X25 MULTI-CONTACT  
      WEHE29-249S424 
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      700.16266.101.0 Berg & Co. GmbH Spanntechnik 
      L+B GEL291AV-02000-G09 
      KNOLL SFL2350/1090-CM3+618  
      KOBOLD Messring GmbH?NKP-1401/1,6 
      HERAEUS 581732 KIT-RF SCREEN,6 INCH QCS/STD 
      UDK20-2PW-PR-S-FP  
      heidenhain RON 785C,ID:355880-30 
      keller?PAA-33X/80794.0-10bar 8-28VDC 
      6SY7000-0AD82 SIEMENS 
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      HAHN+KOLB 53236720  
      Holthausen elektronik GmbH ESW-Compact- 001 (hol600) 
      AC-MOTOREN FBPA132M-4 NO.080401018 
      AirCom MHA-A5  
      Sick 6050134 IMP12-1B5NOVC0F INDUKT NAEHERUNGSSENSOR  
       HOVEN 2050  
      HONEYWELL C7061A1020 
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      rexroth 3DR16P4-5X/200Y/00M R900916667 
      Sick 1056933 AFS60A-BFAA262144 ABSOLUTE ENCODER  
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      Hagglunds SPLL 85A-06 
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      HYDAC CODER EDS348-5-400-000 
      Kistler?C76699 
      Sick 1044463 MZT8-03VPS-KRB MAGN.ZYLINDERSENSOR  
      Leuze electronic GmbH + Co. KG RS4-4/P1,ID-NR.580012  
      KLINGER?INTEC K200-S-FS, DN50, PN40 PART NO.:232005345 
      7000-46061-8020060 Murrelektronik GmbH 
      ERMETO-009 RHD 16-PS  
      HYDROTECHNIK 3403-18-C3.37 
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      HEIDENHAIN MT-101K ,ID-Nr: 361139-02 
      JANE9904 120 16843 With gear box 
      MOOG,,,;() 
      19200100295 19200100295 
      S210.55 S210.56 
      TI100  
      PMA Prozess- und Maschinen-Automation GmbH KS42-100-0000E-000 
      OMS Antriebstechnik OHG?SMT244502 
      RADIO-ENERGIE--RE.0444 L1 B 0,06 CA / JT,Code 
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      Bass (M10 ER20-7;031899 
      Sick 1037515 AFM60B-S1PK032768 ABSOLUTE ENCODER  
      SR SYSTEM-ELEKTRONIK GmbH R-FLAT 19/7-M(R-FLAT 19/3-M) 
      Jungmichel Industrieelektronik GmbH?IT01S 
      7000-78021-9610700 Murr 
      KLH?1640000001 SCHRADER VALVE KLH 
      FIUTEC KHMFF-8-8/1-3-4 DN80  
      SCHUNK SRU+60-W-180-3-M 
      COUPLING/ROTEX GS 38 P 64SHD-H-GS 6.0-32/6.0-35 COUPLING/ROTEX GS 38 P 64SHD-H-GS 6.0-32/6.0-36 
      Werthenbach Hydraulik Antriebstechnik GmbH CG5V 6GW OF M U H5 20 
      FRAKO LKT-12,1-440-D52 K18-0152 
      KUEBLER 8.9080.1831.3001  
      HIRSCHMANN 8*10/100base-TX.TP CABLE.RJ45 
      hydac ZBE08  
      rotalink D63-type 5-cam timer D6305/16H4050  
      kistler 1645C0.6  
      Sick 1060200 DFS60E-BFEK00500 INCREMENTAL ENCODER  
      6x8 TRANSP. PFA IFK-ISOFLUOR 
      Herion 25-512-05 
      7000-46041-8021500 Murr 
      HUBNER EGS 4K 462700  
      MKS 722A 11TCE2FA 0-10TORR 
      jola?COW-LS5 
      WEHTW17H-W9036-065 
      Heinrichs BGN-P305BL000-0-V5E-0-H Serien-Nr. 251981 
      BETA W3-P808H-S1N-B1-K1-X1  
      6SY8102-0AB20 SIEMENS 
      HAWE--KA 24 SKDT/H 0.93-A 3/360-BWH 
      BERTHOLD M/N:LB4430-14-0A-GD  
      HYDAC-4454 HAD4748-H-000 
      HEMOMATIK-0072 BX80A/10-1H 6X 24V 2.5MT 
      RADIO-ENERGIE--REO444N1B0.06CA  
       VSF-35/EP70-230VAC,50/60HZ,0.12KW AUTOMEC  
      FERRATEC 989052 
      brainboxes UC-324,No.481-1258 
      KARL?119438 UV-Spare filter glass (blue) 6302.101 
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      BENDER--IR420-D6W-1 
      70_HGE_690_FV70_A2R Stromag AG 
      6SL3985-6TP00-0AA0 SIEMENS 
      631715-33 substitute for 350093-XXheidenhain 
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      JANERD-121  
      390915 390915 
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      7000-88001-2300500 Murr 
      Gutekunst + Co.KG /PRESSURE SPRING/D-263 2.20*35.80*228.00D  
      JDT?JDT 42*146 
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      klauke?153R 
      Sick 1058202 GRTE18S-N1342 REFLEXIONS-LICHTT.  
      RMG RMG370 DN50 
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       Grundfos CR 5-22 A-FGJ-A-V HQQV; 400V/50Hz 
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      8GM1050-3KK40 8GM1050-3KK41 
      littelfuse 20102000001 
      B&R Industrie-Elektronik GmbH 8MSA3L.E0-V4  
      Balluff GmbH BTL7-G110-M0050-B-S32  
      ITALCOPPIE SENSORI s.r.l.?PT100 junction box, C064021W0000A; 350 * 270 * 205  
       4.3519.00.741(4-20mA,7 pole plug,12m cable) THIES  
      7000-13261-3311000 Murr 
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      Sick 6050226 THTE-1PA521506MZ  
      TC63/750 
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      atos PM-120  
      6DD1684-0BC0 SIEMENS 
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      Sommer GH6280-B/O9 
      Sick 1069340 WL12G-3V2572S05 REFLEXIONS-LICHTSCH.  
      ITAS?NO.A50110453 
      HELVAR?Digidim 458 
       ITOH DENKI CB01P-7 
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      Sick 6048864 LFV200-XXSNHTPM  
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      HEMOMATIK-0047 HMFD1..0=200 0=265 080711 
      WOERNE KF32RF2  
      KTR /COUPLING/ROTEX GS 24 98SHA-GS 2.5-24/2.5-22  
       
      該公司產(chǎn)品分類(lèi): 希而科優(yōu)勢(shì)供應(yīng)

      菲尼根ThermoFinnigan GC/MS/MS

      技術(shù)參數(shù)1.HP 5890 II GC 或者Varian 3400 GC2.CTC A200S Autosampler3.m/z 范圍 10-40004.Xcalibur 計(jì)算機(jī)軟件5.計(jì)算機(jī),顯示器,鍵盤(pán),鼠標(biāo),手冊(cè),電纜主要特點(diǎn) 1.二手翻新設(shè)備2.90天質(zhì)量3.可以選配其他配件4.系統(tǒng)在2002年通過(guò) IQ/OQ  儀器介紹[

      Hep-2細(xì)胞Hep-2細(xì)胞

       Hep-2細(xì)胞  細(xì)胞信息(說(shuō)明書(shū),鑒定書(shū)),售后情況,細(xì)胞活動(dòng)請(qǐng)聯(lián)系 :
      在線QQ:2215398442   郵箱:tongpaibio@126.com  提供耐藥細(xì)胞株,細(xì)胞染色等技術(shù)服務(wù)。

      流式細(xì)胞儀流程:   基本操作: 

      1:收集細(xì)胞:胰酶消化各孔(6孔板),收集到離心管,離心得沉淀; 

      2:用PBS打勻,洗1-2遍,離心,沉淀; 

      CaSki人宮頸癌細(xì)胞 CaSki細(xì)胞  http://www.afzhan.com/st133337/Product_4592206.html

      人宮頸癌細(xì)胞 CaSki細(xì)胞  http://www.afzhan.com/st133337/Product_4405841.html

      3:沉淀的細(xì)胞中加入500uL緩沖液,得懸液; 

      4:加5uL AnnexinV-FITC(細(xì)胞凋亡檢測(cè)試劑盒),混勻; 

      CaSki細(xì)胞 人宮頸癌細(xì)胞  http://www.afzhan.com/st133337/Product_3325174.html

      D341Me人髓母細(xì)胞瘤細(xì)胞 D341Med細(xì)胞傳代  http://www.afzhan.com/st133337/Product_3325180.html

      5:5uLPI混勻;(4)-(6)三組需要避光操作! 

      6:流式細(xì)胞儀測(cè)定。(4×105/孔,6孔板,2mL/孔) 若第二天流式測(cè)量,可加5—10mL70%乙醇,在4℃固定8h以上。 

      Caco-2細(xì)胞 人結(jié)直腸腺癌細(xì)胞  http://www.afzhan.com/st133337/Product_3325168.html

      CaSki細(xì)胞 人宮頸癌細(xì)胞 CaSki細(xì)胞傳代 http://www.afzhan.com/st133337/Product_4632941.html

      制備細(xì)胞凋亡單染管: 活細(xì)胞 

      一半活細(xì)胞+一半死細(xì)胞(高溫滅活-水浴90℃)+FITC(染色活Cell) 

      一半活細(xì)胞+一半死細(xì)胞+PI(PI-碘化丙啶染色核酸,但不能穿透細(xì)胞膜即染色死Cell) 

      該公司產(chǎn)品分類(lèi): 細(xì)胞

      The moorLDI2-BIThe moorLDI2-BI 雙波長(zhǎng)激光多普勒血流儀 創(chuàng)面成像系統(tǒng)

      通過(guò)雙波長(zhǎng)激光,對(duì)兩個(gè)層面的創(chuàng)面血流量進(jìn)行掃描、記錄、分析。    主要用于糖尿病足、周?chē)芗膊、燒傷、休克等微血管血流量相關(guān)疾病的科研、臨床工作。    掃描范圍:5cm x 5cm up to 50cm x 50cm    the moorldi2 laser doppler blood perfusion imagers use a near infra-red laser beam (785nm in the standard moorldi2-ir  imager) or a visible red 633nm laser beam (moorldi2-vr imager). in the moorldi2-2λ  dual wavelength system 633 and 830nm lasers are combined to allow simultaneous imaging with the two wavelengths.    the imagers are able to map tissue blood flow over areas from 5cm x 5cm up to 50cm x 50cm with 256 x 256 pixel resolution; - each pixel being an actual measurement. at high scan rates, over 64,000 measurements are made in less than 5 minutes.    the moorldi2 is suitable for a wide range of clinical and research investigations and measurement of blood flow in the microcirculation. the moorldi2  range features a ccd camera which produces a colour image of the scanned area, making the positioning and comparison of images easier. the moorldi2-hr? highest resolution version is available with resolution to 100 microns.    利用激光多普勒技術(shù)判斷燒傷創(chuàng)面的損傷程度、預(yù)后情況判斷。    臨床實(shí)驗(yàn)證明:bi掃描發(fā)現(xiàn)血流灌注量豐富的創(chuàng)面14天之內(nèi)就會(huì)愈合良好    bi掃描發(fā)現(xiàn)灌注量中等的創(chuàng)面愈合期在14天-21天之間    bi掃描發(fā)現(xiàn)灌注量很低的燒傷創(chuàng)面明確提示醫(yī)生可能需要手術(shù)、愈合期在21天以上    本結(jié)果經(jīng)全球燒傷科臨床醫(yī)學(xué)專(zhuān)家驗(yàn)證    專(zhuān)業(yè)軟件幫助輕松分析監(jiān)測(cè)結(jié)果!    注:影響燒傷愈后的因素很多,請(qǐng)根據(jù)多種情況分析判斷!    introduction    the moorldi2-bi (moorldi2 burns imager) uses the laser doppler technique to predict the healing potential (hp) of a burn wound.    clinical trials have shown that burn wounds heal within 14 days where there is high ld blood flow, between days 14 and 21 with ‘medium’ blood flow, and take longer than 21 days where there is low ld blood flow (the latter usually indicating that surgery is required).    with the introduction of moorldi2-bi  laser doppler imager different regions of a mixed depth burn can be mapped in a single image. a full resolution scan includes 65,536 individual laser doppler measurements and these are displayed alongside a corresponding ccd image, as a colour coded image.    accuracy of moorldi2-bi    since the development of the moorldi, a number of publications have shown the higher accuracy of ldi in burn depth assessment.    niazi et al. (1993) first evaluated the technique of burn depth measurement using ldi. they found that the commonest error (in four out of 17 cases) was overestimation of burn depth by the clinician. in only two cases did they underestimate burn depth. by comparison, when they used ldi to measure burn depth this was correct in all cases (4 superficial dermal and 13 deep-dermal or sub-dermal burns).    pape et al. (2001) published an audit of the use of ldi in the assessment of burn depth. they reported that the ldi allowed accurate assessment of burn depth in 97% of burns, compared with 73% for clinical observation alone. these findings were similar to those reported by monstrey et al. (2001, abstract) who reported ldi accuracy of 93.3–96.6% compared to 61–67% for clinical evaluation, when ldi scanning was performed at day 3 and day 5 post-burn, respectively.    jeng et al. (2001) stated that use of the ldi enabled them to make earlier and more ob-jective determination of the need to excise and graft burns. in a separate study, pape and byrne (2001) presented evidence that the use of ldi avoided unnecessary surgery, resulting in a reduction of both costs and workload.    in 2002 holland et al. also reported that the moorldi  was an accurate method for the prediction of burn wound healing in children (holland et al., 2002). this study involved 57 children with a mean age of 1 year and 10 months. the sensitivity and the specificity of the moorldi  were higher than clinical evaluation:    sensitivity ldi – 0.90; clinical evaluation – 0.66.     specificity ldi – 0.96; clinical evaluation – 0.71 (holland et al., 2002)     equipment required    the moorldi2-bi package includes the moorldi2-bi  scan head, scan controller, touch-screen panel pc, all mounted on a mobile clinical stand that can be used in the ward, operating theatre, consulting room or special laser room. full installation, clinical training and certification is included together with a premium service contract.    summary    the moorldi2-bi can help the burn surgeon by indicating the healing potential (hp) of burn wounds. the current clinical practice is to report burn depth. for example, some burn surgeons use the latarjet classification (first-degree, superficial second-degree, deep second-degree and third-degree). we have introduced the term healing potential (hp) when interpreting moorldi2-bi burn scan images because healing is the recordable outcome measure relevant to large areas of burn, whereas assessment of depth (using biopsy) is only available at discreet points.    a multi-centre study was carried out using the moorldi2-bi  to analyse the ranges of blood flow that correlated with healing within 3 specified time-periods; by post-burn day 14, between post-burn day 14 and 21, and greater than 21 days.    the hp categories are:    hp 14: the wound has the potential to heal within 14 days of the burn injury    hp 14-21: the wound has the potential to heal between 14 to 21 days post burn    hp >21: the wound will take longer than 21 days to heal    the actual healing time of the wound will, however, depend upon other factors that can prolong wound healing (e.g. wound infection or use of inotropes). thus, the moorldi2-bi can provide an indication of the potential of the wound to heal but the actual healing time is dependent upon other clinical, biological and environmental factors.    use of the moorldi2-bi has been shown to avoid unnecessary surgery in patients with superficial dermal wounds and promote early surgery for deep dermal wounds.    the moorldi2  laser doppler blood perfusion imagers use a near infra-red laser beam (785nm in the standard moorldi2-ir imager) or a visible red 633nm laser beam (moorldi2-vr  imager). in the moorldi2-2λ dual wavelength system 633 and 830nm lasers are combined to allow simultaneous imaging with the two wavelengths.    the imagers are able to map tissue blood flow over areas from 5cm x 5cm up to 50cm x 50cm with 256 x 256 pixel resolution; - each pixel being an actual measurement. at high scan rates, over 64,000 measurements are made in less than 5 minutes.    the moorldi2  is suitable for a wide range of clinical and research investigations and measurement of blood flow in the microcirculation. the moorldi2  range features a ccd camera which produces a colour image of the scanned area, making the positioning and comparison of images easier. the moorldi2-hr  highest resolution version is available with resolution to 100 microns.
       

      人前鐵調(diào)素,Pro-Hepc,ELISA,試劑盒,酶免試劑盒

      人前鐵調(diào)素,Pro-Hepc,ELISA,試劑盒,酶免試劑盒

       

          ELISA是酶聯(lián)接免疫吸附劑測(cè)定( Enzyme-Linked Immunosorbnent Assay )的簡(jiǎn)稱。它是繼免疫熒光和放射免疫技術(shù)之后發(fā)展起來(lái)的一種免疫酶技術(shù)。此項(xiàng)技術(shù)自70年代初問(wèn)世以來(lái),發(fā)展十分迅速,目前已被廣泛用于生物學(xué)和醫(yī)學(xué)科學(xué)的許多領(lǐng)域。   

       

      (一) 原理

        ELISA是以免疫學(xué)反應(yīng)為基礎(chǔ),將抗原、牽9體的特異性反應(yīng)與酶對(duì)底物的催化作用相結(jié)合起來(lái)的一種敏感性很高的試驗(yàn)技術(shù)。由于抗原、抗體的反應(yīng)在一種固相載體──聚苯乙烯微量滴定板的孔中進(jìn)行,每加入一種試劑孵育后,可通過(guò)洗滌除去多余的游離反應(yīng)物,從而試驗(yàn)結(jié)果的特異性與穩(wěn)定性。在實(shí)際應(yīng)用中,通過(guò)不同的設(shè)計(jì),具體的方法步驟可有多種。即:用于檢測(cè)抗體的間接法(圖a)、用于檢測(cè)抗原的雙抗體夾心法(圖b)以及用于檢測(cè)小分子抗原或半抗原的抗原競(jìng)爭(zhēng)法等等。比較常用的是ELISA雙抗體夾心法及ELISA間接法。   

       

         ELISA檢測(cè)試劑盒組成:

      1:濃縮洗滌液(50ml20x

      2.標(biāo)本稀釋液(12ml

      3.終止液(12ml

      4.標(biāo)準(zhǔn)品(2管)

      5.酶標(biāo)抗體稀釋液體(12ml

      6.抗體稀釋液(12ml

      7.包被酶標(biāo)板一塊

      8.坐標(biāo)紙一張

      9.封板紙一張

      樣品類(lèi)型(SAMPLE TYPES):

         標(biāo)本必須為液體,不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。1ml的全血可得到0.5ml的血清或血漿。每個(gè)標(biāo)本量收集體積=100ul×檢測(cè)種類(lèi)。取材前須向銷(xiāo)售人員索要說(shuō)明書(shū)。

         標(biāo)本處理:

      收集標(biāo)本前必須清楚要檢測(cè)的成份是否足夠穩(wěn)定。對(duì)收集后當(dāng)天進(jìn)行檢測(cè)的標(biāo)本,儲(chǔ)存在4℃?zhèn)溆,如有特殊原因需要周期收集?biāo)本,將標(biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。避免反復(fù)凍融。標(biāo)本2-8℃可保存48小時(shí),-20℃可保存1個(gè)月。-70度可保存6個(gè)月。部分激素類(lèi)標(biāo)本需添加抑肽酶。

         ◇血清:

      室溫血液自然凝固 10-20 分鐘后,離心 20 分鐘左右( 2000-3000 轉(zhuǎn) / 分)。收集上清。如有沉淀形成,應(yīng)再次離心。

         ◇血漿:

      應(yīng)根據(jù)試劑盒的要求選擇 EDTA 、檸檬酸鈉或肝素作為抗凝劑,加入 10 %( v/v )抗凝劑 0.1M 檸檬酸鈉或 1% heparin 2.0%EDTA.Na2 混合10-20 分鐘后,離心 20 分鐘左右( 2000-3000 轉(zhuǎn) / 分)。仔細(xì)收集上清。如有沉淀形 成,應(yīng)再次離心。

         ◇尿液、胸腹水、腦脊液:

      用無(wú)菌管收集。離心 20 分鐘左右( 2000-3000 轉(zhuǎn) / 分)。仔細(xì)收集上清。如有沉淀形成,應(yīng)再次離心。

         ◇細(xì)胞培養(yǎng)上清:

      檢測(cè)分泌性的成份時(shí),用無(wú)菌管收集。離心 20 分鐘左右( 2000-3000 轉(zhuǎn) / 分)。仔細(xì)收集上清。檢測(cè)細(xì)胞內(nèi)的 成份時(shí),用 PBS PH7.2-7.4 )稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到 100 萬(wàn) /ml 左右。通過(guò)反復(fù)凍融,以使細(xì)胞破壞并放出細(xì)胞內(nèi)成份。離心 20 分鐘左右( 2000-3000 轉(zhuǎn) / 分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。

         ◇組織標(biāo)本:

      切割標(biāo)本后,稱取重量。加入一定量的 PBS ,緩沖液中可加入 1 μ g/L 蛋白酶抑制劑或 50U/ml Aprotinin 抑肽酶)。用手工或勻漿器將標(biāo)本勻漿充分。離心 20 分鐘左右( 2000-3000 轉(zhuǎn) / 分)。仔細(xì)收集上清置于 -20 度或 - 70 度保存,如有必要,可以將樣品濃縮干燥。分裝后一份待檢測(cè),其余冷凍備用。

         檢測(cè)原理:

      采用雙抗體夾心ABC-ELISA

      保存溫度:2-8℃保存6個(gè)月.

      注意事項(xiàng):

      收集標(biāo)本前必須清楚要檢測(cè)的成份是否足夠穩(wěn)定。對(duì)收集后當(dāng)天進(jìn)行檢測(cè)的標(biāo)本,儲(chǔ)存在 4 備用,如有特殊 原因需要周期收集標(biāo)本,將標(biāo)本及時(shí)分裝后放在 -20 -70 條件下保存。避免反復(fù)凍融。標(biāo)本 2 -8 可保存 48 小時(shí), -20 可保存 1 個(gè)月。 -70 度可保存 6 個(gè)月。部分激素類(lèi)標(biāo)本需添加抑肽酶。

         備注:以上為ELISA試劑盒通用說(shuō)明書(shū),不包括特別的試劑盒,具體的請(qǐng)參照每個(gè)產(chǎn)品的說(shuō)明書(shū)

      注:本試劑只用于科研,歡迎來(lái)電咨詢!)如需訂購(gòu)請(qǐng)聯(lián)系24小時(shí)為您服務(wù):

      公司電話:QQ1458738906

       

       

       

      該公司產(chǎn)品分類(lèi): LNP相關(guān)質(zhì)粒 雙熒光素酶質(zhì)粒 腺相關(guān)病毒質(zhì)粒 腺病毒質(zhì)粒 慢病毒質(zhì)粒 干擾質(zhì)粒 骨架載體 質(zhì)粒 沉淀試劑盒 細(xì)胞分選試劑盒 技術(shù)服務(wù) 生化試劑盒 細(xì)胞培養(yǎng)板 細(xì)胞培養(yǎng)皿 細(xì)胞培養(yǎng)瓶 血清移液管 深孔板 普通吸頭 實(shí)驗(yàn)耗材 PCR板

      HE-010西霸士重載連接器

       HE-010重載連接器

      重載連接器——應(yīng)用范圍

      廣泛,如航空、器械設(shè)備等

      重載連接器——種類(lèi)

      針座/插針

      重載連接器——接口類(lèi)型

      AC/DC

      重載連接器——形狀

      矩形

      重載連接器——制作工藝

      其他

      重載連接器——特性

      防水

      重載連接器——接觸件材質(zhì)

      銅鍍銀銅

      重載連接器——絕緣體材質(zhì)

      聚碳酸酯

      重載連接器——芯數(shù)

      3回路,4回路,6回路,10回路,16回路,24回路,32回路,40回路,46回路,64回路

      重載連接器——針數(shù)

      3回路,4回路,6回路,10回路,16回路,24回路32回路,40回路,46回路,64回路

       

      重載連接器型號(hào)

      重載連接器型號(hào)

      重載連接器型號(hào)

      HDC-HE-016-1

      HDC-HE-006-1

      HDC-HA-003/004-1

      HDC-HE-016-2

      HDC-HE-006-2

      HDC-HA-003/004-2

      HDC-HE-016-3 

      HDC-HE-006-3 

      HDC-HA-003/004-3 

      HDC-HE-016-4

      HDC-HE-006-4

      HDC-HA-003/004-4 

      HDC-HE-010

      HDC-HE-024-1

      6回路重載連接器

      HDC-HE-010-1

      HDC-HE-024-2 

      16回路重載連接器

      HDC-HE-010-2

      HDC-HE-024-3

      24回路重載連接器

      HDC-HE-010-3-4 

      HDC-HE-024-4

       HE-010重載連接器

       HE-010重載連接器

      重載連接器的常用規(guī)格:4回路、6回路、10回路、16回路、24回路、32回路、40回路、46回路、64回路。型號(hào)多樣,HAHE、HVEHVES、HEEHD、HDDHSB、HKHM、HQ等系列。HE系列接插件芯件6針接插件公插,有螺釘壓接,彈片壓接,冷壓針壓接等不同的規(guī)格6針接插件母插,有螺釘壓接,彈片壓接,冷壓針壓接等不同的規(guī)格10針接插件公插,有螺釘壓接,彈片壓接,冷壓針壓接等不同的規(guī)格10針接插件母插,有螺釘壓接,彈片壓接,冷壓針壓接等不同的規(guī)格16針接插件公插,有螺釘壓接,彈片壓接,冷壓針壓接等不同的規(guī)格16針接插件母插,有螺釘壓接,彈片壓接,冷壓針壓接等不同的規(guī)格24針接插件公插,有螺釘壓接,彈片壓接,冷壓針壓接等不同的規(guī)格24針接插件母插,有螺釘壓接,彈片壓接,冷壓針壓接等不同的規(guī)格32針接插件公插,有螺釘壓接,彈片壓接,冷壓針壓接等不同的規(guī)格32針接插件母插,有螺釘壓接,彈片壓接,冷壓針壓接等不同的規(guī)格48針接插件公插,有螺釘壓接,彈片壓接,冷壓針壓接等不同的規(guī)格48針接插件母插,有螺釘壓接,彈片壓接,冷壓針壓接等不同的規(guī)格 HE系列接插件是標(biāo)準(zhǔn)型接插件,也是使用最為廣泛的一類(lèi)接插件,通?梢苑譃槁葆斝,彈片型,冷壓針壓接型,雙線端子型。額定電流分別為:5A,10A,16A,40A,100A.電壓一般為500V。有6針,10針,16針,24針,32針,48針的不同規(guī)格。1. 接線形式:螺釘/彈片/冷壓針壓接2. 回路數(shù):648回路/6,10,16,24,322x16),48(2x24)3. 阻燃等級(jí):V0 UL944. 工作電流:16A max5. 工作電壓:500V6. 環(huán)境溫度:-40+1257. 污染等級(jí):3C8. 防護(hù)等級(jí):IP65 。

      該公司產(chǎn)品分類(lèi): 保護(hù)器 HE重載載連接器 HEE系列 HA系列 HE系列 航空插座系列 航空插頭系列 防水插座箱 檢修配電箱 連鎖插座箱 多功能插座箱 機(jī)械隧道專(zhuān)用插座箱 組合插座箱 125A工業(yè)插頭插座 63A工業(yè)插頭插座 32A工業(yè)插頭插座 16A工業(yè)插頭插座 安全光幕 霍爾傳感器 超聲波傳感器

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