旺泉MP系列微型磁力泵(磁力驅(qū)動(dòng)循環(huán)泵)特點(diǎn): 本公司生產(chǎn)該產(chǎn)品歷史,有極小型至大型等多種型號(hào),其外形及工作性能同日本的易威奇(IWAKI)相媲美.選擇流量范圍由每分鐘1公升至120公升,多種不同材質(zhì),可以用來輸送多種腐蝕性的液體。MP系列因其結(jié)構(gòu)小巧,非常適合用作照相沖洗設(shè)備和其它裝置的OEM產(chǎn)品。有軟管、螺紋等連接接口適合各種工業(yè)系統(tǒng)和程序上使用。旺泉泵業(yè)并獲得專利保護(hù)。 最大流量:135L/min 揚(yáng)程:14.3m 主要材質(zhì):GFRPP 功率范圍:3~265W 液溫范圍:0~80℃ 絕無泄漏,結(jié)構(gòu)特殊。本泵可輸送酸、堿、鹽、油品、飲料等介質(zhì),廣泛用于化工、石油化工、精細(xì)化工、染料化工、環(huán)保、水處理、醫(yī)藥、食品等部門。 磁力驅(qū)動(dòng)循環(huán)泵運(yùn)轉(zhuǎn)是在連軸上和葉輪上分別裝配有磁性材料而互相吸引偶合,無需配以傳統(tǒng)機(jī)械軸封,因而是密封的。電動(dòng)機(jī)轉(zhuǎn)動(dòng)扭力是通過主動(dòng)磁及從動(dòng)磁的引力帶動(dòng)葉片工作運(yùn)轉(zhuǎn),F(xiàn)今慣用的磁力驅(qū)動(dòng)泵極大的缺點(diǎn)是在缺液情況下絕對(duì)嚴(yán)禁運(yùn)轉(zhuǎn)否則整機(jī)將毀壞,給使用者增添不必要的麻煩和煩惱。"上海旺泉泵業(yè)"的磁力驅(qū)動(dòng)泵則不必為此擔(dān)心。它采選了一種特別設(shè)計(jì)的特配方陶瓷作軸承,具有極強(qiáng)的耐磨性和抗腐蝕性,使其性能和質(zhì)量安全得以絕對(duì)的。 磁力驅(qū)動(dòng)循環(huán)泵概用無軸封設(shè)計(jì),避免了傳統(tǒng)機(jī)械軸封泵存在的液體泄漏引起的腐蝕后污染的缺陷,是工業(yè)設(shè)備上最理想的液體輸送泵。 以來,在生產(chǎn)流程中為流體輸送和化學(xué)反應(yīng)所裝備的泵,均是軸封式的.即轉(zhuǎn)軸的密封采用機(jī)械密封或填料密封。這種轉(zhuǎn)軸密封裝置不可克服的結(jié)構(gòu)缺點(diǎn)就是泄漏,因?yàn)樗仨氁揽枯斔徒橘|(zhì)或工作介質(zhì)的泄漏來潤滑轉(zhuǎn)軸的密封面,而且隨軸封在高速運(yùn)轉(zhuǎn)中的逐漸磨損,其泄漏量也將隨磨損量的增大而增大,特別是對(duì)易燃、易爆、有毒、有害介質(zhì)的泄漏,既對(duì)環(huán)境造成污染,又危及生產(chǎn)安全和操作人員的健康。為杜絕化工生產(chǎn)領(lǐng)域的泄漏,消除環(huán)境污染,保護(hù)生態(tài)環(huán)境,保障生產(chǎn)安全和操作人員的身體健康.上海旺泉泵業(yè)有限公司結(jié)合本公司及國外技術(shù)與國內(nèi)的大學(xué)的專業(yè)研究院所共同研制開發(fā),成功地推出了旺泉 MP,CQ,CQF,CQB,CQG系列磁力泵無泄漏化工裝備,為我國化工裝備的無泄漏做出了貢獻(xiàn)。 |
PH計(jì)
① pH值測量范圍:0~14.00pH
② mV值測量范圍:0~±1500mV(自動(dòng)極性顯示)
③ pH值測量精度:≤0.05pH
④ mV值測量精度:≤0.5%(讀數(shù))±1個(gè)字
⑤ 輸入阻抗:≥1×1012Ω
⑥ 零點(diǎn)漂移:≤0.03pH/3小時(shí)
大鼠AP核酸內(nèi)切酶-1(APE1)酶聯(lián)免疫分析(ELISA)
試劑盒使用說明書
本試劑僅供研究使用 目的:本試劑盒用于測定大鼠血清、血漿、組織勻漿及相關(guān)液體樣本中AP核酸內(nèi)切酶-1(APE1)的含量。
實(shí)驗(yàn)原理:
本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中大鼠AP核酸內(nèi)切酶-1(APE1)水平。用純化的大鼠AP核酸內(nèi)切酶-1(APE1)捕獲抗體包被微孔板,制成固相抗體,往包被的微孔中依次加入大鼠AP核酸內(nèi)切酶-1(APE1),再與HRP標(biāo)記的檢測抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的大鼠AP核酸內(nèi)切酶-1(APE1)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(OD值),通過標(biāo)準(zhǔn)曲線計(jì)算樣品中大鼠AP核酸內(nèi)切酶-1(APE1)含量。
試劑盒組成:
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注:標(biāo)準(zhǔn)品濃度依次為:16、8、4、2、1、0 ng/mL.
樣本處理及要求:
1. 血清:室溫血液自然凝固10-20分鐘,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清,保存過程中如出現(xiàn)沉淀,應(yīng)再次離心。
2. 血漿:應(yīng)根據(jù)標(biāo)本的要求選擇EDTA或檸檬酸鈉作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清,保存過程中如有沉淀形成,應(yīng)該再次離心。
3. 尿液:用無菌管收集,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清,保存過程中如有沉淀形成,應(yīng)再次離心。胸腹水、腦脊液參照實(shí)行。
4. 細(xì)胞培養(yǎng)上清:檢測分泌性的成份時(shí),用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。檢測細(xì)胞內(nèi)的成份時(shí),用PBS(PH7.2-7.4)稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到100萬/ml左右。通過反復(fù)凍融,以使細(xì)胞破壞并放出細(xì)胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
5. 組織標(biāo)本:切割標(biāo)本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆。?biāo)本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。分裝后一份待檢測,其余冷凍備用。
6. 標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融.
7. 不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
操作步驟
1. 標(biāo)準(zhǔn)品的加樣:設(shè)置標(biāo)準(zhǔn)品孔和樣本孔,標(biāo)準(zhǔn)品孔各加不同濃度的標(biāo)準(zhǔn)品50μL;。
2. 加樣:分別設(shè)空白孔(空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同)、待測樣品孔。在酶標(biāo)包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl(樣品最終稀釋度為5倍)。加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻。
3. 加酶:每孔加入酶標(biāo)試劑100μl,空白孔除外。
4. 溫育:用封板膜封板后置37℃溫育60分鐘。
5. 配液:將20倍濃縮洗滌液用蒸餾水20倍稀釋后備用。
6. 洗滌:小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干。
7. 顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.
8. 終止:每孔加終止液50μl,終止反應(yīng)(此時(shí)藍(lán)色立轉(zhuǎn)黃色)。
9. 測定:以空白孔調(diào)零,450nm波長依序測量各孔的吸光度(OD值)。 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行。
注意事項(xiàng):
1. 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。
2. 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。
3. 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間最好控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。
4. 請每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,最好做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔第一孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計(jì)算時(shí)請最后乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物請避光保存。
7. 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
8. 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9. 本試劑不同批號(hào)組分不得混用。
10. 如與英文說明書有異,以英文說明書為準(zhǔn)。
計(jì)算:
以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),
在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD
值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度;再乘以稀釋
倍數(shù);或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)
準(zhǔn)曲線的直線回歸方程式,將樣品的OD值
代入方程式,計(jì)算出樣品濃度,再乘以稀釋
倍數(shù),即為樣品的實(shí)際濃度。
(此圖僅供參考)
試劑盒性能:
1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上。
2.批內(nèi)變異系數(shù)與批間變異系數(shù)應(yīng)分別小于10%和15% 。
檢測范圍:
0.5 ng/mL - 16 ng/mL
靈敏度:
最低檢測濃度小于0.1 ng/mL
保存條件及有效期:
1.試劑盒保存: 2-8℃。
2.有效期: 6個(gè)月
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Drug Names
Generic Name:Rat apurinic;apyrimidinic endonuclease 1 (APE1) ELISA Kit.
Purpose
This kit allows for the determination of APE1 concentrations in Rat serum, plasma, tissue homogenates and other biological fluids.
Principle of the assay
The kit assay Rat APE1 level in the sample, use Purified Rat APE1 antibody to coat microtiter plate wells, make solid-phase antibody, then add APE1 to the wells, Combined antibody which With HRP labeled, become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
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Note: Standard concentration was followed by:
16、8、4、2、1、0 ng/mL.
Specimen requirements
1. serum- coagulation at room temperature 10-20 mins,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4. cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS(PH7.2-7.4), Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5. Tissue samples- After cutting samples, check the weight,add PBS(PH7.2-7.4), Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS(PH7.4), Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.add enzyme:Add HRP-Conjugate reagent 100μl to each well, except blank well.
4.Incubate: After closing plate with Closure plate membrane ,incubate for 60 min at 37℃.
5.Configurate liquid: 20-fold wash solution diluted 20-fold with distilled water and reserve.
6.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
7.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
8.Stop the reaction:Add Stop Solution 50μl to each well, Stop the reaction(the blue color change to yellow color).
9.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2. washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4. if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8. All samples, washing buffer and each kind of reject should according to infective material process.
9. Do not mix reagents with those from other lots.
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Calculate
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Assay range
0.5 ng/mL - 16 ng/mL
Sensitivity
The minimum detectable dose is typically less than 0.1 ng/mL
Storage and validity
1.Storage: 2-8℃.
2.validity: six months.
詳細(xì)說明:
一、 應(yīng)用范圍(可分析周期表中所有金屬元素和部分非金屬元素)
KEP公司是世界上的現(xiàn)場儀表制造公司,主要生產(chǎn)流量產(chǎn)品、信號(hào)轉(zhuǎn)換器、過程控制器、指示器、計(jì)數(shù)器、流量計(jì)算機(jī)、批量控制器等,也涉及自動(dòng)控制系統(tǒng)。其產(chǎn)品經(jīng)濟(jì)耐用,操作方便簡單。廣泛應(yīng)用于石油、化工、電力、冶金、制藥、造紙、食品等行業(yè)。
系列如下:AH57,AHK07,AK,AMB,AMK,AMP,AW,B15,B16,B18,BA8ANA,BA8IO7,BAT,BC,BCA,BCR,BT2,CH,CTF,DPF,E,ETM,FLO,H37,H57,HB17,HC77,HK07,INT6*,KAL,KE,L,MASS,ML,MM,MT,PC,ST,PD,TR,W15,W16,ZOIDDA,ZOIDDB,ZOIDDC,XHV等等
功能說明 :顯示範(fàn)圍:2~ 6 位數(shù)設(shè)定範(fàn)圍:1~999999字型尺寸:0.56"LED 2或4位數(shù) 0.4" LED 5或6位數(shù) 計(jì)數(shù)輸入:有接點(diǎn)輸入:25HZ 無接點(diǎn)輸入:3KHZ輸出方式:1 Relay output(1a,b AC 250OV/5A),0.1~5 seconds復(fù)歸方式:外部手動(dòng)&自動(dòng)復(fù)歸輸入信號(hào):NPN open collector (或PNP輸入)電源電壓:AC 110V/ 220V ±15% 50HZ / 60HZ信號(hào)電源:DC12V±5 MAX消耗電力:3.3VA耐溫溫度:-10℃ ~ + 50℃ 35% ~ 85%RH尺寸規(guī)格:72mm × 72mm × 145mm (盤面開孔:68mm×68mm)
供應(yīng)鉑鑒管道彎曲試驗(yàn)機(jī) PVC管彎曲試驗(yàn)機(jī)廠家 金屬管彎曲試驗(yàn)機(jī)制造商
產(chǎn)品性能:GWQ-50鋼管彎曲試驗(yàn)機(jī)是對(duì)金屬管進(jìn)行平面彎曲試驗(yàn)的專用設(shè)備。該設(shè)備是依據(jù)GB/T244-2008 ISO8491:1998(E)《金屬管彎曲試驗(yàn)方法》開發(fā)的新型產(chǎn)品。該產(chǎn)品廣泛應(yīng)用于金屬管廠和管道企業(yè),以檢驗(yàn)金屬管的彎曲性能。 設(shè)備精度可靠,使用方便,限位可靠,使用壽命長,是目前最完善的彎曲試驗(yàn)機(jī)。 鋼管彎曲試驗(yàn)機(jī)產(chǎn)品主要技術(shù)指標(biāo): 1、型號(hào): GWQ-502、最大彎曲鋼管直徑 40-50 3、正彎角度設(shè)定 180° 4、反彎角度設(shè)定 180°5、工作盤轉(zhuǎn)速 ≤20°/S 6、鋼管彎曲試驗(yàn)機(jī)工作盤直徑(mm) 680 7、功率(kW) 1.5 8、外觀: 應(yīng)符合GB/T2611要求9、成套性: 符合標(biāo)準(zhǔn)要求10、供電電源: 220V,50Hz11、主機(jī)外形尺寸(mm) 1500×800×2100 12、重量(kg) 約1000 試驗(yàn)機(jī)的工作條件:1、在室溫10℃~35℃范圍內(nèi),相對(duì)濕度不大于80%;2、鋼管彎曲試驗(yàn)機(jī)需在穩(wěn)固的基礎(chǔ)或工作臺(tái)上,正確安裝;3、在無振動(dòng)、無腐蝕性介質(zhì)及無較強(qiáng)定磁場干擾的的環(huán)境中;4、電源電壓的波動(dòng)范圍不應(yīng)超過額定電壓的 +/-10%。
鋼管彎曲試驗(yàn)機(jī) 管道彎曲試驗(yàn)機(jī) PVC管彎曲試驗(yàn)機(jī) 金屬管彎曲試驗(yàn)機(jī) 橡膠管不銹鋼管彎曲試驗(yàn)機(jī)