精品国产高清免费第一区二区三区，亚洲国产成人成上人色，欧美理论片a手机在线观看，人人澡人人澡人人看欧美，四虎国产精品久久久久久网址，久久伍月精品网，久久久久国产激情老熟女，国产精品久久久久久久久久

PT壓力變送器（pressure sensor）

我公司（svloadcell@hotmail.com）主要經(jīng)營壓力傳感器（load cell）、稱重傳感器,測力傳感器,拉力傳感器,張力傳感器,壓力變送器（pressure sensor），稱重系統(tǒng)（weighing system）,配料稱重系統(tǒng),罐體稱重系統(tǒng),料斗稱重系統(tǒng),電子稱，汽車稱重儀，軸重儀及附件的生產(chǎn)廠家。公司的產(chǎn)品線主要分為：應(yīng)變式測力／稱重傳感器、應(yīng)變式扭矩／多分力傳感器及壓力變送器、應(yīng)變式流體壓力傳感器及變送器和傳感器用高精度電阻式應(yīng)變計(jì)等產(chǎn)品。其中有各種型式的稱重傳感器,如橋式的,剪切梁式的,單點(diǎn)式,柱式的,輪輻式的,板式的,S型的,平行梁式的,同時(shí)也提供OEM,可能根據(jù)客戶要求自行設(shè)計(jì)各類稱重測力傳感器.

　　近年來，又開發(fā)了各種稱重測試臺(tái)、日用衡器用傳感器及電子衡器、高精度稱重傳感器用應(yīng)變計(jì)等新產(chǎn)品系列。

電　　話： 0510-88056151

傳　　真： 0510-88056151

郵件:svloadcell@hotmail.com

郵　　編： 214215

公司網(wǎng)站： http://www.load-cell.com.cn

CC1-805 vpc90度低溫水浴

CC1-805 vpc90度低溫水浴油浴加熱、制冷功率大，溫度范圍：-90℃--300℃，帶有強(qiáng)有力的壓力泵和吸收泵。 CC1-805 vpc90度低溫水浴全部采用內(nèi)外不銹鋼的槽，壽命長。有單加熱和同時(shí)可加熱和制冷油浴型號(hào)，制冷功率最大到7KW@20℃,加熱功率到4KW。該產(chǎn)品系列可以選擇加“矯正插件”或“減少體積插件”用做矯正油浴，水浴和反應(yīng)釜專用控溫設(shè)備。 VPC系列產(chǎn)品可選擇“ComBox”(到MAMUR)的信號(hào)傳導(dǎo)系統(tǒng)/Protocol以及外循環(huán)壓力控制器。安全：所有產(chǎn)品根據(jù)DIN12876標(biāo)準(zhǔn)設(shè)計(jì)生產(chǎn)，帶有可調(diào)節(jié)過熱保護(hù)和液位監(jiān)視器。所有產(chǎn)品都可以使用CC1,CC2或者CC3的面板相互替換。 CC1-805 vpc90度低溫水浴技術(shù)參數(shù)：內(nèi)槽體積(L)：5 工作電壓（V）：400 溫度范圍(℃)：-80～100 ℃ 制冷功率（KW）： 20 ℃時(shí)：0.5 0 ℃時(shí)：0.5 -20 ℃時(shí)：0.4 -40 ℃時(shí)：0.3 -60 ℃時(shí)：0.3 深度（mm）：150 開口尺寸（mm）：120＊110 外形尺寸（mm）：410＊480＊720 訂貨號(hào)：693.0031

PHG-2081型工業(yè)在線PH計(jì)-上海博取儀器有限公司

PHG-2081型工業(yè)PH計(jì)

上海博取儀器有限公司是專業(yè)從事水質(zhì)分析儀器、自動(dòng)化儀器儀表的開發(fā)、設(shè)計(jì)、制造、銷售及服務(wù)于一體的綜合性企業(yè)。PHG-2081型工業(yè)PH計(jì)性能穩(wěn)定，操作簡單，價(jià)格合理。

PHG-2081型工業(yè)pH計(jì)是我公司的pH分析儀，本表為高智能化在線連續(xù)監(jiān)測儀，由傳感器和二次表兩部分組成�？膳淙龔�(fù)合或兩復(fù)合電極，以滿足各種使用場所。

PHG-2081型工業(yè)PH計(jì)儀器特點(diǎn)：

智能化： pHG-2081工業(yè)pH計(jì)采用高精度AD轉(zhuǎn)換和單片機(jī)微處理技術(shù)，能完成pH值和溫度的測量、溫度自動(dòng)補(bǔ)償、儀表自檢等多種功能。

性：元器件集成到一塊線路板上, 沒有了復(fù)雜的功能開關(guān)、調(diào)節(jié)旋鈕和電位器。

雙高阻輸入：采用器件，雙高阻輸入阻抗高達(dá)10¹²Ω，抗干擾能力強(qiáng)；

溶液接地：消除地回路的干擾。

電流隔離輸出：采用光電耦合隔離技術(shù)，抗干擾能力強(qiáng)，可遠(yuǎn)傳；

通訊接口：可方便聯(lián)入計(jì)算機(jī)進(jìn)行監(jiān)測和通訊。

自動(dòng)溫度補(bǔ)償：在0～99.9℃自動(dòng)溫度補(bǔ)償。

防水防塵設(shè)計(jì)：防護(hù)等級(jí)IP65，適宜戶外使用。

中文顯示、中文菜單、中文記事：采用菜單結(jié)構(gòu)，類似微機(jī)操作，操作簡單，操作步驟全程中文提示，可不用說明書，即可方便完成。

多參數(shù)同屏顯示：在同一屏幕上顯示pH值、輸入mV數(shù)（或輸出電流）、溫度、時(shí)間和狀態(tài)等。

PHG-2081型工業(yè)PH計(jì)技術(shù)指標(biāo)：

1、測量范圍： pH值 0～14.00pH，精度 0.01pH；

溫度 0～99.9℃，精度 0.1℃；

電位值 –1999.9～+1999.9mV，精度 0.1mV；

2、自動(dòng)溫度補(bǔ)償范圍： 0～99.9℃ ，25℃為基準(zhǔn)；

3、被測水樣： 0～99.9℃，0.6MPa；

4、電子單元自動(dòng)溫度補(bǔ)償誤差：±0.03pH；

5、電子單元重復(fù)性誤差： ±0.02pH；

6、穩(wěn)定性： ±0.02pH/24h；

7、輸入阻抗： ≥10¹²Ω ；

8、時(shí)鐘精度： ±1分/月；

9、電流隔離輸出： 0～10mA（負(fù)載<1.5 kΩ ）, 4～20 mA（負(fù)載<750 Ω ）；

10、輸出電流誤差： ≤±1%FS ；

11、數(shù)據(jù)存儲(chǔ)數(shù)量： 1個(gè)月（1點(diǎn)/5分鐘）；

12、高低報(bào)警繼電器： AC220V，3A；

13、RS485通訊接口； RS485或232（選配）

14、電源： AC220V±22V ，50Hz±1Hz；

15、防護(hù)等級(jí)： IP65；

16、外形尺寸： 146（長）×146（寬）×108（深）mm，

開孔尺寸： 138×138 mm；

17、重量： 0.8kg ；

18、工作條件：環(huán)境溫度 0～60℃ 相對濕度 <85%；

19、可配三復(fù)合或兩復(fù)合電極。

該公司產(chǎn)品分類： COD分析儀氟離子檢測儀磷酸根/硅酸根分析儀鈉度計(jì) 酸堿濃度計(jì) 濁度儀余氯分析儀電導(dǎo)率儀 PH計(jì) 溶氧儀

57300-USPME 固相微萃取頭

SPME 固相微萃取頭，3支/盒應(yīng)用：用于揮發(fā)性物質(zhì)（MW60-275）SPME 固相微萃取頭簡介：美國Supelco公司專利產(chǎn)品－固相微萃�。⊿olid Phase Micro Extraction），1994年獲美國匹茲堡分析儀器會(huì)議R&D100項(xiàng)革新大獎(jiǎng)，是一種應(yīng)現(xiàn)代儀器的要求而產(chǎn)生的樣品前處理新技術(shù)，幾乎克服了以往一些傳統(tǒng)樣品處理技術(shù)的所有缺點(diǎn)，集采樣、萃取、濃縮、進(jìn)樣于一體，便于攜帶，真正實(shí)現(xiàn)樣品的現(xiàn)場采集和富集，能夠與氣相、氣相-質(zhì)譜、液相、液相-質(zhì)譜儀聯(lián)用，有手動(dòng)或自動(dòng)兩種操作方式，讓更多的分析工作者從重復(fù)、煩瑣的操作中解脫出來。廣泛應(yīng)用于環(huán)保及水質(zhì)處理、臨床藥理、公安案件分析、制藥、化工、國防等領(lǐng)域。固相微萃�。⊿PME）非常小巧,狀似一只色譜注射器，由手柄（Holder）和萃取頭或纖維頭（Fiber）兩部分構(gòu)成。萃取頭是一根外套不銹鋼細(xì)管的1cm長、涂有不同色譜固定相或吸附劑的熔融石英纖維頭，纖維頭在不銹鋼管內(nèi)可自由伸縮，用于萃取、吸附樣品；手柄用于安裝或固定萃取頭，可永久使用。SPME 固相微萃取頭的典型應(yīng) 用：* 表面活性劑* 環(huán)境水樣* 食品、香精、香料* 法庭樣品* 血、尿和體液中聚合物和固樣中痕量雜質(zhì)（頂空方式）* 藥物中殘留溶劑* 氣體硫化物及揮發(fā)物（VOC）

該公司產(chǎn)品分類：水質(zhì)分析儀/多參數(shù)水質(zhì)分析儀生化試劑實(shí)驗(yàn)室儀器燒杯實(shí)驗(yàn)室用具泵色譜耗材及配件樣品前處理儀器及耗材

Inspector Alert α、β、γ和X射線檢測儀

儀器介紹

◆采用一只蓋革-彌勒計(jì)數(shù)管來測定α、β、γ和X射線輻射 ◆“安全第一”（Safety First）的校準(zhǔn)功能能夠避免校準(zhǔn)人員的輻射接觸 ◆檢測儀符合歐洲CE認(rèn)證要求

主要特點(diǎn)

◆內(nèi)置鹵素淬滅劑GM探測器，對α、β射線源的靈敏度很高 ◆四位液晶顯示，可選擇mR/hr、CPM、mSv/hr、CPS或Total/Timer等單位 ◆總計(jì)數(shù)/定時(shí)器功能對輕微污染進(jìn)行定時(shí)的精確檢測，定時(shí)時(shí)間可選擇1分鐘－－ 24小時(shí)

技術(shù)參數(shù)

◆測量范圍：mR/hr(毫倫/小時(shí))：0.001—110.0，CPM（每分鐘計(jì)數(shù)）：0—300,000 μSv/hr(微希伏/小時(shí))：0.01—1,100，CPS（每秒鐘計(jì)數(shù)）：0—5,000，總計(jì)數(shù)： 1—9,999,000 ◆效率：Sr-90(546kev,2.3MeV βmax)約75% C-14(156kev βmax)約11% Bi-210(1.2MeV βmax)約64% Am-241(5.5MeV α)約36% ◆靈敏度：3500CPM/ mR/hr（對于Cs-137） ◆精度：±15% ◆溫度范圍：-10℃－－-+50℃ ◆電源：1節(jié)9V堿性電池，電池壽命 200小時(shí)◆尺寸重量：150×80×30mm 350克（含電池）

應(yīng)用領(lǐng)域

◆探測和測定表面沾污◆在操作放射性核素時(shí)監(jiān)測可能存在的放射性暴露量◆調(diào)查環(huán)境污染◆測定惰性氣體及其它低能放射性核素◆建筑裝飾材料放射測定射線危害：低劑量的放射性射線輻射（天然背景輻射的變化范圍），對人體無害或風(fēng)險(xiǎn)甚低，但達(dá)到一定劑量則會(huì)對人體有害，可引起癌癥、白內(nèi)障、不孕癥、突變、萎縮效應(yīng)、壽命減短,甚至死亡

應(yīng)用：

偵測放射性射線，以采取相應(yīng)防護(hù)措施。海關(guān)和邊境巡邏，政府執(zhí)法部門，檢疫檢驗(yàn)，應(yīng)急事故處理，核電廠、銀行、政府、實(shí)驗(yàn)室等部門安全巡查，醫(yī)學(xué)廢料處理，消防隊(duì)，采礦業(yè)，科學(xué)實(shí)驗(yàn)，個(gè)人保護(hù)，連續(xù)監(jiān)測

參考信息（來自中國輻射防護(hù)研究院）

居民的劑量限值為每年1mSv。即0.114μSv/hr。

放射性職業(yè)人員劑量限值為每年20mSv，但任何一年不能超過50mSv。

該公司產(chǎn)品分類：核素識(shí)別儀便攜式中子測量儀便攜式β/γ/中子譜儀電離室巡測儀表面污染測量儀多功能輻射測量儀個(gè)人劑量報(bào)警儀便攜式放射性監(jiān)測儀

AN218004DWDM光譜分析儀AN218004 [1526.05—1563.86 nm] SC/PC

DWDM光譜分析儀AN218004 [1526.05—1563.86 nm] SC/PC DWDM光譜分析儀特征：·PLC 設(shè)備· DWDM（96通道）測量解決方案· 同一時(shí)間自動(dòng)測量 CWDM-96通道 (波長 & 功率) · CWDM 彩色圖文掃描· 保存和調(diào)用300數(shù)據(jù)· 軟件可以自行保存數(shù)據(jù)· 自動(dòng)關(guān)機(jī)DWDM光譜分析儀技術(shù)參數(shù)：

技術(shù)參數(shù)
通道數(shù)量	96	通道間隔	50GHz, 100GHz
通道頻率	196.45~191.7THz	最大輸入功率	500mW; 27dBm
帶寬	1526.05~1563.86 nm	光源接頭	SC/PC Standard
測量速度	4 Sec. (all 96ch.)	電池	鋰聚合物電池, 1800毫安時(shí)，3.7伏
測量范圍	+10~-40dBm	電池工作時(shí)長	充滿電后單次使用620分鐘
測量精度	± 1.0dB @ -40 dBm	電流消耗(Max)	0.25A
顯示屏分辨率	0.01dB	電力消耗	0.925W
顯示單位	dB, dBm,nm,THz	顯示	3.5” TFT-LCD, 16bit color, 240*320
重量	0.6 kg	尺寸	1969540 mm
溫度 (環(huán)境條件)	-20 to +55 °C (操作環(huán)境)	濕度 (最大無冷凝)	95% (操作環(huán)境)
溫度 (環(huán)境條件)	-35 to +65°C (儲(chǔ)存環(huán)境)	濕度 (最大無冷凝)	85% (儲(chǔ)存環(huán)境)

http://www.yach.com/Instrument.html 更多光學(xué)儀器儀表

該公司產(chǎn)品分類：衛(wèi)星天線慣性導(dǎo)航系統(tǒng) 天線微波射頻儀器通訊儀器儀表光通訊儀器儀表光學(xué)儀器儀表光通訊儀器儀表

GM0120PQ1MNN美國米頓羅GM系列機(jī)械隔膜式計(jì)量泵

美國米頓羅GM系列機(jī)械隔膜式計(jì)量泵/GM0120米頓羅加藥泵/蘇州加藥泵

計(jì)量泵GM0050 /米頓羅GM0050/米頓羅計(jì)量泵GM0050/蘇州GM0050計(jì)量泵

GM0090米頓羅/GM0090計(jì)量泵/米頓羅計(jì)量泵GM0090/蘇州GM0090計(jì)量泵

米頓羅GM0120 /計(jì)量泵GM0120 /米頓羅計(jì)量泵GM0120/蘇州GM0120計(jì)量泵

米頓羅GM0170 /計(jì)量泵GM0170 /米頓羅計(jì)量泵GM0170/蘇州GM0170計(jì)量泵

GM0500米頓羅/GM0500計(jì)量泵/米頓羅計(jì)量泵GM0500/蘇州GM0500計(jì)量泵

主要型號(hào)包括：GM0050 GM0090 GM0120 GM0170 GM0240 GM0330 GM0400 GM0500

特點(diǎn)：

平滑脈沖設(shè)計(jì)，無沖擊 ? 沖程在靜態(tài)及動(dòng)態(tài)條件下均可調(diào)節(jié)

多種材質(zhì)及泵頭結(jié)構(gòu)可選 ? 可選配電動(dòng)沖程控制器

機(jī)械驅(qū)動(dòng)PTFE膜片 ? 最大液體溫度40 ℃＊

手動(dòng)/ 自動(dòng)沖程調(diào)節(jié)可選 ? 穩(wěn)態(tài)精度 +/- 2%(10%~100%)

沖程可調(diào)0~100%(自動(dòng)/手動(dòng)) ? 最大吸程4m水柱＊

可選配電機(jī)控制器實(shí)現(xiàn)外部信號(hào)自動(dòng) 控制， ? 最大吸入壓力：20m 水柱

電機(jī)頻率0~100%可調(diào)(GM泵自動(dòng)控制方式)

米頓羅機(jī)械隔膜泵，GM和GB系列兩個(gè)系列。泵頭材質(zhì)為PVC，PP，SS316，高粘度等可選擇，適合多種場合液體介質(zhì)的輸送。流量范圍從2.25L到1800L均有。

可選雙隔膜泵頭結(jié)構(gòu)

建議維護(hù)周期：自動(dòng)控制方式選擇

電動(dòng)沖程控制器接受外部控制信號(hào)，調(diào)節(jié)計(jì)量泵沖程長度，從而改變計(jì)量泵輸出流量。

GM系列計(jì)量泵選擇ST型。 GB系列計(jì)量泵選擇ECC型。

變頻控制器：接受外部控制信號(hào)，調(diào)節(jié)計(jì)量泵沖程頻率，從而改變計(jì)量泵輸出流量。

供電：220V/50HZ 單向

380V/50HZ 三相控制信號(hào)：4~20mA 電機(jī)控制器(VARIPULSE):

僅用于GM系列計(jì)量泵，用于控制三相電機(jī)，改變沖程頻率，從而調(diào)節(jié)計(jì)量泵輸出流量。

V 型－同計(jì)量泵一體式安裝 VR型－同計(jì)量泵分體式安裝

主要型號(hào)包括：GM0050 GM0090 GM0120 GM0170 GM0240 GM0330 GM0400 GM0500

上海 | 江蘇 | 浙江 | 安徽 | 福建 | 江西 | 山東 | 山西| 湖北 | 湖南 | 廣東 | 廣西 | 海南 | 重慶 | 四川 | 貴州 | 云南

蘇州米頓羅機(jī)械隔膜計(jì)量泵

1)*系列型號(hào)編碼	流量* L/h	壓力 MPa	沖程 spm
GM0002PL1MNN GM0005PL1MNN GM0010PL1MNN	2.25 4.5 9	1.2 1.2 1.2	36 72 144
GM0025PL1MNN GM0050PL1MNN	25 50	1.2 1.0	72 144
GM0090PQ1MNN GM0120PQ1MNN GM0170PQ1MNN GM0240PP1MNN	85 115 170 235	0.7 0.7 0.7 0.7	72 72 144 144
GM0330PP1MNN GM0400PP1MNN GM0500PP1MNN	315 400 500	0.5 0.5 0.5	144 144 180

上海 | 江蘇 | 浙江 | 安徽 | 福建 | 江西 | 山東 | 山西| 湖北 | 湖南 | 廣東 | 廣西 | 海南 | 重慶 | 四川 | 貴州 | 云南

特點(diǎn)：

•平滑脈沖設(shè)計(jì)，無沖擊 •沖程在靜態(tài)及動(dòng)態(tài)條件下均可調(diào)節(jié)

•多種材質(zhì)及泵頭結(jié)構(gòu)可選 •可選配電動(dòng)沖程控制器

•機(jī)械驅(qū)動(dòng)PTFE膜片 •最大液體溫度40 ℃＊

•手動(dòng)/ 自動(dòng)沖程調(diào)節(jié)可選 •穩(wěn)態(tài)精度±2%(10%~100%)

•沖程可調(diào)0~100%(自動(dòng)/手動(dòng)) •最大吸程4m水柱＊

•可選配電機(jī)控制器實(shí)現(xiàn)外部信號(hào)自動(dòng) 控制，•最大吸入壓力：20m 水柱

電機(jī)頻率0~100%可調(diào)(GM泵自動(dòng)控制方式)

•可選雙隔膜泵頭結(jié)構(gòu)

•建議維護(hù)周期：≤1.0MPa 4000h 　　　　　　

≤0.7MPa 8000h

自動(dòng)控制方式選擇

•電動(dòng)沖程控制器接受外部控制信號(hào)，調(diào)節(jié)計(jì)量泵沖程長度，從而改變計(jì)量泵輸出流量。

GM系列計(jì)量泵選擇ST型。 GB系列計(jì)量泵選擇ECC型。

•變頻控制器：接受外部控制信號(hào)，調(diào)節(jié)計(jì)量泵沖程頻率，從而改變計(jì)量泵輸出流量。

供電：220V/50HZ 單向

380V/50HZ 三相

控制信號(hào)：4~20mA

•電機(jī)控制器(VARIPULSE):

僅用于GM系列計(jì)量泵，用于控制三相電機(jī)，改變沖程頻率，從而調(diào)節(jié)計(jì)量泵輸出流量。

V 型－同計(jì)量泵一體式安裝

VR型－同計(jì)量泵分體式安裝

該公司產(chǎn)品分類：塞瓦計(jì)量泵新道茨NEWDOSE 日本安智邁計(jì)量泵德普羅名特計(jì)量泵 SDP斯得浦計(jì)量泵美帕斯菲達(dá)計(jì)量泵意大利EMEC愛米克計(jì)量泵 TTbest精密計(jì)量泵意大利道茨計(jì)量泵意大利SEKO計(jì)量泵米頓羅計(jì)量泵力高計(jì)量泵愛力浦計(jì)量泵計(jì)量泵

CSB-E04488h人環(huán)磷酸腺苷(cAMP)ELISA Kit

1Human cyclic adenosinemonophosphate（cAMP）Elisa KitCatalog No. CSB-E04488h（96T） This immunoassay kit allows for the in vitro quantitative determination of humancAMP concentrations in serum, plasma. Expiration date six months from the date of manufacture FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.2INTRODUCTIONCyclic adenosine monophosphate （cAMP, cyclic AMP or 3＇-5＇-cyclicadenosine monophosphate） is a second messenger important in manybiological processes. cAMP is derived from adenosine triphosphate （ATP）and used for intracellular signal transduction in many different organisms,conveying the cAMP-dependent pathway.cAMP is synthesised from ATP by adenylyl cyclase located on the innerside of the plasma membrane. Adenylyl cyclase is activated by a range ofsignaling molecules through the activation of adenylyl cyclase stimulatory G（Gs）-protein-coupled receptors ａnd inhibited by agonists of adenylylcyclase inhibitory G （Gi）-protein-coupled receptors. Liver adenylyl cyclaseresponds more strongly to glucagon, ａnd muscle adenylyl cyclase respondsmore strongly to adrenaline.cAMP decomposition into AMP is catalyzed by the enzymephosphodiesterase.cAMP is a second messenger, used for intracellular signal transduction,such as transferring the effects of hormones like glucagon ａnd adrenaline,which cannot pass through the cell membrane. It is involved in theactivation of protein kinases ａnd regulates the effects of adrenaline andglucagon. It also regulates the passage of Ca2+ through ion channels.3PRINCIPLE OF THE ASSAYThe microtiter plate provided in this kit has been pre-coated with agoat-anti-mouse IgG. Standards or samples are then added to theappropriate microtiter plate wells with a HRP-conjugated cAMP andantibody preparation specific for cAMP ａnd incubated. Then substratesolutions are added to each well. The enzyme-substrate reaction isterminated by the addition of a sulphuric acid solution ａnd the color changeis measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Theconcentration of cAMP in the samples is then determined by comparing theO.D. of the samples to the standard curve.DETECTION RANGE0.08pmol/ml-250 pmol/ml. The standard curve concentrations used for theELISA’s were 250 pmol/ml, 50 pmol/ml, 10 pmol/ml, 2 pmol/ml, 0.4 pmol/ml,0.08 pmol/ml.SPECIFICITYThis assay recognizes human cAMP No significant cross-reactivity orinterference was observed.4MATERIALS PROVIDEDReagent QuantityAssay plate 1Standard1 x 0.25 ml（5000pmol/ml）HRP-conjugate 1（1000 x stock solution）Antibody 1（1000 x stock solution）HRP-conjugate Diluent 1 x 6 mlAntibody Diluent 1 x 6 mlNeutralizing Reagent 1 x 6 mlWash Buffer1 x 15 ml（10×concentrate）Substrate A 1 x 10 mlSubstrate B 1 x 10 mlStop Solution 1 x 6 mlOTHER SUPPLIES REQUIRED1. Deionized or distilled water.2. Concentrated HCl.3. Precision Pipets for volumes between 5μ l ａnd 1000μ l.4. Repeater Pipets for dispensing 50μ l ａnd 200μ l.5. Disposable breakers for diluting buffer concentrates.6. Graduated cylinders.7. A microplate shaker.8. Adsorbent paper for blotting.9. Microplate reader capable of reading at 450 nm, preferably withcorrection between 570 ａnd 590 nm.5STORAGE1. For long-term best results, store stocks of the Standard ,Antibody andHRP-conjugate at -80℃. All other components of this kit are stable at4°C until the kit＇s expiration date.SAMPLE COLLECTION AND STORAGEThis ELISA is compatible with cAMP samples that have been treated withhydrochloric acid to stop endogenous phosphodiesterase activity. Samplesin this matrix can be measured directly without evaporation or furthertreatment.Tissue samples should be frozen in liquid nitrogen. The tissue should beground to a fine powder under liquid nitrogen in a stainless steel mortar.After the liquid nitrogen has evaporated, weigh the frozen tissue andhomogenize in 10 volumes of 0.1M HCl. Centrifuge at > 600 x g at roomtemperature. The samples can then be diluted in the 0.1M HCl.Cells grown in tissue culture media can be treated with 0.1M HCl after firstremoving the media. Incubate for 10 minutes ａnd visually inspect the cellsto verify cell lysis. If adequate lysis has not occurred incubate for a further10 minutes ａnd inspect. Centrifuge at 600 x g at room temperature, thenuse the supernatant directly in the assay. Cell or tissue lysis can beenhanced by adding 0.1% to 1% Triton x-100 to the 0.1M HCl prior to use.When used in this concentration range, the detergent will not interfere with6the binding portion of the assay, however there will be a modest increase inthe optical density. Samples containing Triton should be evaluated against astandard curve diluted in the same for the most accurate determination.Cyclic AMP in the media can be measured after treating 1 mL of thesupernatant media with 10 μ L of concentrated hydrochloric acid.Centrifuge at 600 x g at room temperature. The supernatants can then beused directly in the assay.Procedural Notes1. Allow all reagents to warm to room temperature for at least 30 minutesbefore opening.2. Pre-rinse the pipet tip with reagent ,use fresh pipet tips for eachsample,standard ａnd reagent.3. Pipet standards ａnd samples to the bottom of the wells.4. Add the reagents to the side of the well to avoid contamination.5. The kit uses break-apart microtiter strips,which allow the user tomeasure as many samples as desired.Unused wells must be keptdesiccated at 4℃ in the sealed bag provided,The wells should be usedin the frame provided.6. Prior to addition of substrate ensure that there is no residual washbuffer in the wells .Any remaining wash buffer may cause variationin assay resultes.REAGENT PREPARATIONBring all reagents to room temperature before use.71. Wash Buffer If crystals have formed in the concentrate, warm to roomtemperature ａnd mix gently until the crystals have completely dissolved.Dilute 15 ml of Wash Buffer Concentrate into deionized to prepare 150ml of Wash Buffer.2. HRP-conjugate working solution: Centrifuge the vial right before use.Dilute the HRP-conjugate 1000x stock solution （take 6μl） with theprovided dilution buffers （6 ml）.3. Antibody working solution: Centrifuge the vial right before use. Dilutethe Antibody 1000x stock solution （take 6μl） with the provided dilutionbuffers （6 ml）.4. Standards: Centrifuge the vial right before use. Allow the 5,000pmol/mL cAMP standard solution to warm to room temperature. Labelsix tubes #1 through #6. Pipet 475 μL 0.1M HCl into tube #1 ａnd 400 μL0.1M HCl into tubes #2-6. Add 25 μL of the 5,000 pmol/mL standard totube #1. Vortex thoroughly. Add 100 μL of tube #1 to tube #2 andvortex thoroughly. Continue this for tubes #3 through #6. Theconcentration of cAMP in tubes #1 through #6 will be 250, 50, 10, 2, 0.4,and 0.08 pmol/mL respectively. Diluted standards should be usedwithin 30 minutes of preparation. Label one tube as the ZeroStandard/NSB tube. Pipet 600μl 0.1M HCl into this tube.ASSAY PROCEDUREBring all reagents to room temperature for at least 30 minutes prioropening.ALL standards ａnd samples should be run in duplicate.Add the reagen directly to the samples ａnd vortex for 2 seconds immediatelyafter the addition.81. Refer to the Assay Layout Sheet to determine the number of wells to beused ａnd put any remaining wells with the desiccant back into thepouch ａnd seal the ziploc .Store unused wells at 4℃.2. Pipet 50 μL of the Neutralizing Reagent into each well, except theTA（Total Activity）ａnd Blank wells.3. Pipet 100 μL of 0.1M HCl into the NSB（None Specific Binding）ａnd theBo （0 pmol/mL Standard） wells.4. Pipet 100 μL of Standards into the appropriate wells.5. Pipet 100 μL of the Samples into the appropriate wells.6. Pipet 50 μL of 0.1 M HCl into the NSB wells.7. Pipet 50 μL of HRP-conjugate working solution into each well exceptthe TA ａnd Blank wells.8. Pipet 50 μL of Antibody working solution into each well, except theBlank, TA ａnd NSB wells.9. Incubate the plate at room temperature for 2 hours on a plate shaker at250~500 rpm.10. Empty the contents of the wells ａnd wash by adding 400 μL of washsolution to every well. Repeat the wash 2 more times for a total of 3washes.11. After the final wash, empty or aspirate the wells, ａnd firmly tap the plateon a lint free paper towel to remove any remaining wash buffer.12. Add 5 μL of the HRP-conjugate working solution to the TA wells.913. Add 200 μL of the Substrate solution to every well. Incubate at roomtemperature for 5~30 minutes without shaking. A gradient of blue colorshould become visible during the incubation period. （Substrate A andB should be mixed together in equal volumes within 15 minutes ofuse. Protect from light.）14. Add 50 μL of Stop Solution to every well. This stops the reaction andthe plate should be read immediately.15. Blank the plate reader against the Blank wells, read the optical densityat 450 nm （for HRP）, preferably with correction between 570 ａnd 590nm. If the plate reader is not able to be blanked against the Blankwells, manually subtract the mean optical density of the Blank wellsfrom all readings.1 0CALCULATION OF RESULTSSeveral options are available for the calculation of the concentration ofcAMP in the samples. The X-axis is the concentration of cAMP for thestandards. The Y-axis is either the Average Net Optical Density or thePercent Bound.1. Calculate the average Net Optical Density （OD） bound for each standardand sample by subtracting the average NSB OD from the average ODbound:Average Net OD = Average Bound OD － Average NSB OD2. Calculate the binding of each pair of standard wells as a percentage ofthe maximum binding wells （Bo）, using the following formula:3. Using Logit-Log paper plot Average Net OD or Percent Bound（B/Bo） versus concentration of cAMP for the standards. Theconcentration of cAMP in the unknowns can be determined byinterpolation.1 1Typical Standard CurvesThese curves must not be used to calculate cAMP concentrations; eachuser must run a standard curve for each assay ａnd version used.SensitivitySensitivity was calculated by determining the average optical density boundfor ten wells run with the Bo, ａnd comparing to the average optical densityfor ten wells run with Standard #5. The detection limit was determined asthe concentration of cAMP measured at two standard deviations from thezero along the standard curve.Non-Acetylated VersionMean OD for Bo = 0.685±0.003Mean OD for Standard #5 = 0.604±0.010Delta Optical Density（0-0.08pmol/ml） = 0.0812 SD＇s of the Zero Standard = 0.006Sensitivity =￣0.006/0.081×0.4pmol/ml = 29.6 fmol/mL1 2LinearityA sample containing 16.0 pmol/mL cAMP was serially diluted 7 times 1:2 inthe 0.1M HCl ａnd measured. The data was plotted graphically as actualcAMP concentration versus measured cAMP concentration. The lineobtained had a slope of 1.000 with a correlation coefficient of 0.999.Cross ReactivitiesThe cross reactivities for a number of related compounds were determinedby competition ELISA assays. Potential cross reactants were dissolved inthe kit Assay Buffer at concentrations from 500,000 to 500 pmol/mL. Thesesampleswere then measured in the cAMP assay, ａnd the measured cAMPconcentration at 50% B/Bo calculated. The % cross reactivity wascalculated by comparison with the actual concentration of cross reactant inthe sample ａnd expressed as a percentage.Compound Cross ReactivitycAMP 100%AMP <0.0001%ATP <0.0001%cGMP <0.0001%GMP <0.0001%GTP <0.0001%cUMP <0.0001%CTP <0.0001%

該公司產(chǎn)品分類：科研試劑

HS706C.T.P4S.DUL軸承FAG

HS706C.T.P4S.DUL軸承 HS706C.T.P4S.DUL軸承價(jià)格 HS706C.T.P4S.DUL軸承尺寸 HS706C.T.P4S.DUL軸承報(bào)價(jià) 精密角接觸球軸承樣本圖

訂購原裝FAG軸承請撥打021-3653 9288【FAG HS706C.T.P4S.DUL軸承】尺寸規(guī)格參數(shù)：軸承型號(hào)：HS706C.T.P4S.DUL軸承軸承系列：精密角接觸球軸承軸承品牌：FAG軸承內(nèi)徑：6 外徑：17 厚度：6 動(dòng)載荷（Cr）：1.56 靜載荷（Cor）：0.7 脂潤滑限速：150000油潤滑限速：220000凈重kg：0.01 產(chǎn)品描述：鋼球,15度,樹脂保持架,P4級(jí),開式，對裝自由配組上海東晟軸承有限公司提供 HS706C.T.P4S.DUL軸承的軸向壓力、滾粒的數(shù)目、動(dòng)態(tài)負(fù)荷、靜態(tài)負(fù)荷、直徑、替代型號(hào)等技術(shù)參數(shù)，我公司代理經(jīng)營美國FAG軸承，瑞典SKF軸承，日本 NSK、NTN、IKO軸承，德國FAG、INA軸承、NADELLA軸承等名優(yōu)進(jìn)口軸承,如果您對 6 mm×17 mm×6 mm FAG HS706C.T.P4S.DUL軸承有疑問，請聯(lián)系我們獲取更多HS706C.T.P4S.DUL軸承的最新信息。TEL:021-3653 9288 我公司專業(yè)現(xiàn)貨供應(yīng)精密角接觸球軸承 HS706C.T.P4S.DUL軸承，我們?yōu)槟扑]以下型號(hào)： B7004-E-T-P4S軸承 R18/16-8 23096BKD1 NN3026軸承 PHS3EC HC7004-C-T-P4S軸承 7036CD/DT W036 6320-2Z軸承 4T-HM911242/HM911210 6301-2RZ軸承 6016-RS1 2207K NA4826軸承 SD544 FAG HS706C.T.P4S.DUL軸承我公司還為您推薦FAGHS706C.T.P4S.DUL軸承廣泛應(yīng)用于: 齒輪泵, 手動(dòng)泵, 釘跟機(jī), 玻璃反應(yīng)釜, 移印機(jī), 食品藥品包裝機(jī), 化纖機(jī)械, 塑料板材設(shè)備, 氣流干燥設(shè)備, 等領(lǐng)域。EGBZ0812-E40軸承 http://www.fag.sh.cn/zhoucheng/EGBZ0812-E40.htmlEGBZ0814-E40軸承 http://www.fag.sh.cn/zhoucheng/EGBZ0814-E40.htmlC081008軸承 http://www.fag.sh.cn/zhoucheng/C081008.htmlBCE85軸承 http://www.fag.sh.cn/zhoucheng/BCE85.htmlK20X26X12軸承 http://www.fag.sh.cn/zhoucheng/K20X26X12.htmlL812147/L812111軸承 http://www.timken.sh.cn/TIMKEN/L812147_L812111.htmlL812148/L812111軸承 http://www.timken.sh.cn/TIMKEN/L812148_L812111.html29590/29520軸承 http://www.timken.sh.cn/TIMKEN/29590_29520.html29590/29522軸承 http://www.timken.sh.cn/TIMKEN/29590_29522.html395A/394軸承 http://www.timken.sh.cn/TIMKEN/395A_394.htmlEWP211軸承 http://www.nsk.sh.cn/bearings/EWP211.htmlEWP212軸承 http://www.nsk.sh.cn/bearings/EWP212.htmlEWP305軸承 http://www.nsk.sh.cn/bearings/EWP305.htmlAX4.5120155軸承 http://www.nadella.sh.cn/bearings/AX4.5120155.html

該公司產(chǎn)品分類：軸承