產(chǎn)品介紹 |
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技術(shù)指標 |
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主要技術(shù)參數(shù):
u 最大試驗力:600KN
u 試驗臺離地高度:300mm
u 有效試驗空間:1200mm×1200mm
u 加載方式:液壓
u 控載方式:手動無極可調(diào)。(全自動加載 選配)
u 活塞行程:300mm(400mm選配)
u 試驗力值精度范圍:≤±1%(≤±1%選配) 節(jié)范圍:0.1~45KN/s(手動無極調(diào)速)
u 載荷指示精度:0.1KN(0.1KN 選配) 加載速度調(diào)
u 變形測量分辨率:液晶數(shù)顯表自動采集0.01mm (選配),變形測量范圍:0.0-20mm (選配)
u 加載介質(zhì):68#抗磨液壓油
u 電機功率:1.2KW ,電壓:380V/220V
u 主機尺寸:1600mm×1200mm×1400mm(長×寬×高)
u 主機重量:3200Kg
電氣連接: | 表面貼裝/板載式 |
類型: | 磁場測量 |
特點: | 操作簡單,使用方便 |
供電電源: | 12VDC |
輸出: | 20±4mV/V |
精確度: | <50µV/V(磁滯) |
工作溫度范圍: | -40℃~+125℃ |
量程: | - |
典型應(yīng)用: | 磁場強度測量,磁學(xué)成像流量計 |
YSA2系列智能型萬能式斷路器用于控制和保護低壓配電網(wǎng)絡(luò)。一般安裝在低壓配電柜中做主開關(guān)起總保護作用。其技術(shù)性能已達到了當代國際上同類型產(chǎn)品水平!鼋涣黝~定電流630~6300A;■短路分斷能力80KA~120KA(值);■額定工作電壓AC690V及以下;■具有3級和4級;■抽屜式和固定式■可倒進線安裝;■多種智能控制器,提供不同功能;■執(zhí)行IEC60947-2/GB14048.2的標準;
超卓牌廠家ZDLSY電動Y型疏水閥產(chǎn)品簡介:
ZDLSY電動Y型疏水閥是根據(jù)熱電廠設(shè)備疏水而專業(yè)設(shè)計生產(chǎn)的新一代蒸汽疏水閥,該產(chǎn)品接近直線的流通設(shè)計,減小沖蝕,提高壽命。閥芯、閥座采用整體硬質(zhì)合金,關(guān)閉內(nèi)漏,檢修方便等特點,根據(jù)控制系統(tǒng)或調(diào)節(jié)儀表的信號,切斷或開啟閥門,從而實現(xiàn)對被控參數(shù)的使用要求。因此它廣泛應(yīng)用于火電廠等行業(yè)的遠程控制。
技術(shù)參數(shù):
產(chǎn)品口徑:DN15~50;
產(chǎn)品壓力:0.6-6.4MPa;
連接方式:螺紋、法蘭;
適用介質(zhì):水、油品、氣、蒸汽等;
產(chǎn)品材質(zhì):鑄鋼、不銹鋼、合金鋼等。
連接尺寸:
公稱通徑DN | 20 | 25 | 32 | 40 | 50 | 65 | 80 | 100 |
流量系數(shù) | 5 | 8 | 12 | 17 | 29 | 43 | 70 | 110 |
額定行程mm | 16 | 25 | 40 | |||||
公稱壓力PN | 150LB(2.0MPa)--1500LB(25.0MPa) | |||||||
工作溫度 | -445 | |||||||
信號范圍 | 40-20mADC 200VAC 380VAC | |||||||
電源 | 220VAC 50HZ 380VAC 50HZ 60HZ | |||||||
允許泄漏量 | 近似0泄露 | |||||||
靈敏度 | (0.5-5%)F.S |
ZDLSY電動Y型疏水閥訂貨須知:一、①ZDLSY電動Y型疏水閥產(chǎn)品名稱與型號②ZDLSY電動Y型疏水閥口徑③ZDLSY電動Y型疏水閥是否帶附件以便我們的為您正確選型。二、若已經(jīng)由設(shè)計單位選定超卓公司的ZDLSY電動Y型疏水閥型號,請按ZDLSY電動Y型疏水閥型號直接向我司銷售部訂購。三、當使用的場合非常重要或環(huán)境比較復(fù)雜時,請您盡量提供設(shè)計圖紙和詳細參數(shù),由我們的超卓閥門專家為您審核把關(guān)。
專業(yè)定制國內(nèi)外各廠家微波消解罐內(nèi)罐
已有國產(chǎn),何必進口。
讓微波消解罐不在高“貴”
1.內(nèi)罐采用高純實驗級進口增強改性處理TFM材料或PFA材料;
2.與國產(chǎn)PTFE相比:除PTFE的所有優(yōu)點以外,TFM還具有一些特性改進:具有更低的本底保證,高溫高壓下抗變形性、耐滲透性、可恢復(fù)性更好,是PTFE的兩倍,外觀更接近于白玉色;
3.使用溫度為260℃,極限甚至可達300℃;
4.特殊研發(fā)生產(chǎn)工藝,保證特別廠家(如CEM)的超長罐的光潔度,可定制各廠家各規(guī)格微波消解儀內(nèi)罐,配套原廠微波消解儀使用;
5.經(jīng)特殊加工工藝加工,優(yōu)化加工工藝,確保極低的背景值(空白值),生產(chǎn)廠家的加工工藝也能影響您的實驗結(jié)果;
與原廠相比我們的特別之處:
1.同等優(yōu)質(zhì)的進口原料,價格是進口的一半甚至三分之一,并非所有的國產(chǎn)產(chǎn)品,不值得信賴,做到真正的物美價廉;
2.我們所加工的內(nèi)杯能與您的儀器通用,產(chǎn)品實驗數(shù)據(jù)與原裝進口一樣優(yōu)秀,使用壽命更長;
3.我們提供配套CEM各種型號微波消解儀使用的內(nèi)罐,有全部采用TFM材質(zhì),有蓋子采用TFM材質(zhì),有蓋子是透明PFA材質(zhì),蓋體和墊片均為TFM材質(zhì),及全PTFE材質(zhì),供客戶選擇;
4.我們是一家能定制各個廠家儀器標配的內(nèi)罐,同時我們承諾,產(chǎn)品質(zhì)量與原廠一致,性價比好,可以加工CEM 各種型號如MARS5、 MARS6 、安東帕、邁爾斯通、上海屹堯、山東海能等各廠家。
我公司可以定制國內(nèi)外各個廠家(如CEM、耶拿、邁爾斯通、北京祥鵠、上海新儀、山東海能、北分瑞利、上海屹堯、安東帕)的微波消解儀內(nèi)罐,我公司特殊研發(fā)生產(chǎn)工藝保證特別廠家(如CEM MARS5、MARS6、XPRESS)的超長罐的光潔度。消解完成后,可以配套我公司的趕酸電熱板進行趕酸、消解處理。
南京瑞尼克科技開發(fā)有限公司
銷售部:季溪 QQ:182 6176 783
電話:025-8559 7772 手機:138 138 88374
傳真:025-5899 4772 E-mail:13813888374@163.com
韓國SANG-A相阿3A接頭 Y型三通接頭.
PY04 PY06 PY08 SANG-A PY10 PY12 PW0604 PW0804 PW0806 PW1006 SANWOPW1008 PW1208 PW1210 PY5/32 PY3/16 PY1/4 PY5/16 PY3/8 PY1/2 PW3/16-5/32 DANHI PW1/4-5/32 PW1/4-3/16 PW5/16-5/32 SANWO PW5/16-1/4 PW3/8-1/4 DANHI PW3/8-5/16 PW1/2-5/16 PW1/2-3/8 PYJ04 PYJ06 SANG-APYJ08 PYJ10 PYJ12 PYJ5/32 PYJ3/16 PYJ1/4 PYJ5/16 PYJ3/8 PYJ1/2 PWJ0604 SANG-A PWJ0806 PWJ1008 PWJ1210 PWJ1/4-5/32 PWJ5/16-1/4 PWJ3/8-5/16 PWJ1/2-3/8 PW0403C PW0604C PW1/8C-03C PW5/32C-1/8C DANHI PW1/4C-5/32C PWJ04-03C PWJ06-04C PWJ1/8C-03C SANWO PWJ5/32C-1/8C PWJ1/4C-5/32C
【Hypersil系列液相色譜柱的參數(shù)說明】 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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溫州瑞昕儀器有限公司
溫州瑞昕儀器有限公司是從事工業(yè)用檢測儀器、理化分析儀器、計量儀器產(chǎn)品的銷售、服務(wù)、維修一體的公司。歡迎您的咨詢訂購,聯(lián)系方式:
公司電話:0 5 7 7-6 6 8 8 6 0 1 7
傳真:0 5 7 7-6 6 8 8 6 0 1 9
郵箱:rxin17@163.com
網(wǎng)址:http://www.rxin17.com/
聯(lián)系人:
陳進碗 手機:1 3 7 5 8 7 6 8 8 1 3 QQ:124677072
人蛔蟲IgM抗體酶聯(lián)免疫分析(ELISA)
試劑盒使用說明書
本試劑僅供研究使用 目的:本試劑盒用于測定人血清,血漿及相關(guān)液體樣本中蛔蟲IgM抗體的含量。
實驗原理:
本試劑盒應(yīng)用雙抗原夾心法測定標本中人蛔蟲IgM抗體水平。用純化的人蛔蟲IgM抗原包被微孔板,制成固相抗原,往包被抗原的微孔中依次加入蛔蟲IgM抗體,再與HRP標記的蛔蟲IgM抗原結(jié)合,形成抗原-抗體-酶標抗原復(fù)合物,經(jīng)過洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的蛔蟲IgM抗體呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(OD值),通過標準曲線計算樣品中人蛔蟲IgM抗體濃度。
試劑盒組成:
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樣本處理及要求:
1. 血清:室溫血液自然凝固10-20分鐘,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清,保存過程中如出現(xiàn)沉淀,應(yīng)再次離心。
2. 血漿:應(yīng)根據(jù)標本的要求選擇EDTA或檸檬酸鈉作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清,保存過程中如有沉淀形成,應(yīng)該再次離心。
3. 尿液:用無菌管收集,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清,保存過程中如有沉淀形成,應(yīng)再次離心。胸腹水、腦脊液參照實行。
4. 細胞培養(yǎng)上清:檢測分泌性的成份時,用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。檢測細胞內(nèi)的成份時,用PBS(PH7.2-7.4)稀釋細胞懸液,細胞濃度達到100萬/ml左右。通過反復(fù)凍融,以使細胞破壞并放出細胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
5. 組織標本:切割標本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆谩吮救诨笕匀槐3?/span>2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。分裝后一份待檢測,其余冷凍備用。
6. 標本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應(yīng)盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應(yīng)避免反復(fù)凍融.
7. 不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
操作步驟
1. 標準品的稀釋與加樣:在酶標包被板上設(shè)標準品孔10孔,在、第二孔中分別加標準品100μl,然后在、第二孔中加標準品稀釋液50μl,混勻;然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標準品稀釋液50μl,混勻;然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標準品稀釋液50ul,混勻;混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標準品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標準品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉。(稀釋后各孔加樣量都為50μl,濃度分別為60pg/ml,40pg/ml ,20 pg/ml,10 pg/ml , 5pg/ml。
2. 加樣:分別設(shè)空白孔(空白對照孔不加樣品及酶標試劑,其余各步操作相同)、待測樣品孔。在酶標包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl(樣品最終稀釋度為5倍)。加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻。
3. 溫育:用封板膜封板后置37℃溫育30分鐘。
4. 配液:將30(48T的20倍)倍濃縮洗滌液用蒸餾水30(48T的20倍)倍稀釋后備用。
5. 洗滌:小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干。
6. 加酶:每孔加入酶標試劑50μl,空白孔除外。
7. 溫育:操作同3。
8. 洗滌:操作同5。
9. 顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.
10. 終止:每孔加終止液50μl,終止反應(yīng)(此時藍色立轉(zhuǎn)黃色)。
11. 測定:以空白空調(diào)零,450nm波長依序測量各孔的吸光度(OD值)。 測定應(yīng)在加終止液后15分鐘以內(nèi)進行。
注意事項:
1. 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。
2. 濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果。
3. 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其性,以避免試驗誤差。一次加樣時間控制在5分鐘內(nèi),如標本數(shù)量多,推薦使用排槍加樣。
4. 請每次測定的同時做標準曲線,做復(fù)孔。如標本中待測物質(zhì)含量過高(樣本OD值大于標準品孔孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計算時請乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物請避光保存。
7. 嚴格按照說明書的操作進行,試驗結(jié)果判定必須以酶標儀讀數(shù)為準.
8. 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9. 本試劑不同批號組分不得混用。
10. 如與英文說明書有異,以英文說明書為準。
計算:
以標準物的濃度為橫坐標,OD值為縱坐標,
在坐標紙上繪出標準曲線,根據(jù)樣品的OD
值由標準曲線查出相應(yīng)的濃度;再乘以稀釋
倍數(shù);或用標準物的濃度與OD值計算出標
準曲線的直線回歸方程式,將樣品的OD值
代入方程式,計算出樣品濃度,再乘以稀釋
倍數(shù),即為樣品的實際濃度。
(此圖僅供參考)
試劑盒性能:
1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.92以上。
2.批內(nèi)與批見應(yīng)分別小于9%和15%
檢測范圍:
1 pg/ml -70 pg/ml
保存條件及期:
1.試劑盒保存:;2-8℃。
2.期:6個月
FOR RESEARCH USE ONLY
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Drug Names
Generic Name:Human ascariasis IgM antibody (ASC-IgM) ELISA Kit.
Purpose
This kit allows for the determination of ASC-IgM concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human ASC-IgM level in the sample,use Purified Human ASC-IgM antigen to coat microtiter plate wells, make solid-phase antigen, then add ASC-IgM to wells, Combined ASC-IgM antigen which With HRP labeled , become antigen - antibody - enzyme- antigen complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ASC-IgM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
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| (20ml×20 fold) ×1bottle | (20ml×30 fold) | |
Specimen requirements
1. serum- coagulation at room temperature 10-20 mins,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4. cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS(PH7.2-7.4), Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5. Tissue samples- After cutting samples, check the weight,add PBS(PH7.2-7.4), Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS(PH7.4), Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separately. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 60pg/ml,40pg/ml ,20 pg/ml,10 pg/ml , 5pg/ml)
2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2. washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4. if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8. All samples, washing buffer and each kind of reject should according to infective material process.
9. Do not mix reagents with those from other lots.
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Calculate
This chart for reference only
Assay range
1 pg/ml -70 pg/ml
Storage and validity
1.Storage: 2-8℃.
2.validity: six months.